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Significant progress has been achieved in the last 15\u00a0years, since the initial reports surfaced from G<\/jats:styled-content>ermany, USA<\/jats:styled-content> and Israel of the C<\/jats:styled-content>yHV<\/jats:styled-content>\u20103 virus, in terms of pathology and detection. However, relatively few studies have been carried out in understanding viral replication and propagation. Antibody\u2010based affinity has been used for detection of C<\/jats:styled-content>yHV<\/jats:styled-content>\u20103 in enzyme\u2010linked immunosorbent assay and PCR<\/jats:styled-content>\u2010based techniques, and immunohistological assays have been used to describe a C<\/jats:styled-content>yHV<\/jats:styled-content>\u20103 membrane protein, termed ORF<\/jats:styled-content>81. In this study, monoclonal antibodies linked to N<\/jats:styled-content>\u2010hydroxysuccinimide (NHS<\/jats:styled-content>)\u2010activated spin columns were used to purify C<\/jats:styled-content>yHV<\/jats:styled-content>\u20103 and host proteins from tissue samples originating in either C<\/jats:styled-content>yHV<\/jats:styled-content>\u20103 symptomatic or asymptomatic fish. The samples were next analysed either by polyacrylamide gel electrophoresis (PAGE<\/jats:styled-content>) and subsequently by electrospray ionization coupled to mass spectrometry (ESI<\/jats:styled-content>\u2010MS<\/jats:styled-content>) or by ESI<\/jats:styled-content>\u2010MS<\/jats:styled-content> analysis directly after purification. A total of 78 host proteins and five C<\/jats:styled-content>yHV<\/jats:styled-content>\u20103 proteins were identified in the two analyses. These data can be used to develop novel control methods for CyHV<\/jats:styled-content>\u20103, based on pathways or proteins identified in this study.<\/jats:p>","DOI":"10.1111\/jfd.12073","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T15:58:46Z","timestamp":1359043126000},"page":"721-733","source":"Crossref","is-referenced-by-count":14,"title":["Antibody screening identifies 78 putative host proteins involved in C<\/scp>yprinid herpesvirus 3 infection or propagation in common carp, C<\/scp>yprinus carpio<\/i>\u00a0L<\/scp>"],"prefix":"10.1111","volume":"36","author":[{"given":"M","family":"Gotesman","sequence":"first","affiliation":[{"name":"Clinical Division of Fish Medicine University of Veterinary Medicine Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Soliman","sequence":"additional","affiliation":[{"name":"Clinical Division of Fish Medicine University of Veterinary Medicine Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"El\u2010Matbouli","sequence":"additional","affiliation":[{"name":"Clinical Division of Fish Medicine University of Veterinary Medicine Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.40.53"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00146-07"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2010.11.004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.1.94"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-13"},{"key":"e_1_2_6_7_1","first-page":"74","article-title":"Detection of koi herpesvirus (KHV) after re\u2010activation in persistently infected common carp (Cyprinus carpio L.) using non\u2010lethal sampling 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