{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T17:50:24Z","timestamp":1774288224030,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T00:00:00Z","timestamp":1374451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Approximately 8\u00a0weeks after a chlorine insult associated with the city water supply, shortnose sturgeon,<jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>cipenser brevirostrum<\/jats:italic>(L.), from one group presented with small (3\u20134\u00a0mm) irregular foci of cutaneous pallor that involved the dorsocranial integument with progressive ulceration of the nascent lesions. Various bacterial organisms were isolated from the cutaneous lesions, but not from the internal viscera. Histologically, the nuclei of the intralesional and perilesional epidermal cells often exhibited margination of the chromatin that resulted in a homogenous, pale, amphophilic, tinctorial quality of the nucleoplasm consistent with a herpesvirus infection. In addition, rare lamellar epithelial cells were prominently enlarged due to an abundant, dense, basophilic cytoplasm characteristic of an iridovirus infection. Inoculation of cutaneous lesion and kidney, spleen, liver sample pools from affected shortnose sturgeon onto white sturgeon spleen (<jats:styled-content style=\"fixed-case\">WSS<\/jats:styled-content>\u20102) cell line induced cytopathic effect characterized by syncytia formation. Ultrastructural analysis of infected<jats:styled-content style=\"fixed-case\">WSS<\/jats:styled-content>\u20102 cells revealed viral particles with a characteristic herpesvirus morphology. Intranuclear hexagonal capsids had a diameter of 95\u2013108\u00a0nm, and enveloped particles present in the cytoplasm of infected cells had a diameter of 176\u2013196\u00a0nm. This is the first report of a herpesvirus and a possible iridovirus\u2010like infection in shortnose sturgeon.<\/jats:p>","DOI":"10.1111\/jfd.12147","type":"journal-article","created":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T14:04:45Z","timestamp":1374501885000},"page":"141-147","source":"Crossref","is-referenced-by-count":19,"title":["Case report: concurrent herpesviral and presumptive iridoviral infection associated with disease in cultured shortnose sturgeon,<i><scp>A<\/scp>cipenser brevirostrum<\/i>(L.), from the<scp>A<\/scp>tlantic coast of<scp>C<\/scp>anada"],"prefix":"10.1111","volume":"37","author":[{"given":"S E","family":"LaPatra","sequence":"first","affiliation":[{"name":"Research Division Clear Springs Foods, Inc. Buhl ID USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J M","family":"Groff","sequence":"additional","affiliation":[{"name":"Department of Pathology Microbiology and Immunology School of Veterinary Medicine University of California Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Keith","sequence":"additional","affiliation":[{"name":"Aquaculture Management Division Fisheries and Oceans Canada Courtenay BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W E","family":"Hogans","sequence":"additional","affiliation":[{"name":"Atlantic Reference Center Huntsman Marine Science Center St. Andrews NB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Groman","sequence":"additional","affiliation":[{"name":"Aquatic Diagnostic Services Atlantic Veterinary College University of Prince Edward Island Charlottetown PE Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,22]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"29","article-title":"Identification of an iridovirus in Russian sturgeon (Acipenser guldenstadi) from northern Europe","volume":"18","author":"Adkison M.A.","year":"1998","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.5962\/p.357178","article-title":"Rare and endangered fishes and marine mammals of Canada: COSEWIC fish and marine mammal sub\u2010committee status reports IX","volume":"107","author":"Campbell R.R.","year":"1993","journal-title":"Canadian Field\u2010Naturalist"},{"key":"e_1_2_5_4_1","unstructured":"ConteP.S. DoroshovS.I. LutesP.B.&StrangeE.M. (1988)Hatchery manual for the white sturgeon (Acipenser transmontanus Richardson) with application to other North American Acipenseridae. Cooperative Extension University of California Division of Agriculture and Natural Resources Publ. 3322 104pp."},{"key":"e_1_2_5_5_1","unstructured":"DadswellM.J. TaubertB.D. SquiersT.S. MarchetteD.&BuckleyJ.(1984)Synopsis of Biological Data on Shortnose Sturgeon Acipenser brevirostrumLeSueur 1818. NOAA Technical Report NMFS 14 October 1984. FAO Fisheries Synopsis No. 140."},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.1232"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao008039"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao011049"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8659(1991)120<0528:TCLFWS>2.3.CO;2"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao012075"},{"key":"e_1_2_5_11_1","volume-title":"Animal Tissue Techniques","author":"Humason G.L.","year":"1979"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1577\/H05-002.1"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1577\/H07-028.1"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/08997659.2011.545697"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(94)90036-1"},{"key":"e_1_2_5_16_1","unstructured":"LaPatraS.E. ParkerB.L. GroffJ.M. EngelkingH.M. KaufmanJ.&MunnR.J. (1998)Epidemiology of viral infections in white sturgeon from the Pacific Northwest. Proceedings of the 49th Pacific Northwest Fish Culture Conference Boise Idaho. pp27\u201331."},{"key":"e_1_2_5_17_1","volume-title":"Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens","author":"Thoesen J.C.","year":"1994"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao022199"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao032173"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12147","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12147","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12147","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T05:17:45Z","timestamp":1715750265000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,22]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/jfd.12147"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12147","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,22]]}}}