{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:05:33Z","timestamp":1790823933477,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,8,16]],"date-time":"2013-08-16T00:00:00Z","timestamp":1376611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research University Grants Scheme (RUGS), Universiti Putra Malaysia","award":["01-01-11-1136RU"],"award-info":[{"award-number":["01-01-11-1136RU"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u2018Gold standard\u2019 <jats:styled-content style=\"fixed-case\">OIE<\/jats:styled-content> reference <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (<jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content>) in freshwater ornamental fish from <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>alaysia. From total of 210 ornamental fish samples representing 14 species, <jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content> was detected in 36 samples representing 5 fish species. All positive cases did not show any clinical signs of <jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content>. Three restriction enzymes analyses showed that the fish were infected by identical strains of the same virus species within <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>egalocytivirus<\/jats:italic> genus. Major capsid protein (<jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>) genes of 10 <jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content> strains were sequenced and compared with 9 other reference nucleotide sequences acquired from <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>en<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>ank. Sequence analysis of <jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content> gene showed that all strains detected in this study were closely related to the reference <jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content> with nucleotide sequence identity that was ranging from 99.8% to 100%. In addition, phylogenetic analysis of <jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content> gene revealed that viruses from genus <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>egalocytivirus<\/jats:italic> can be divided into three genotypes: genotype 1 include reference <jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content> and all other strains that were detected in this study, genotype 2 include viruses closely related to red sea bream iridovirus (<jats:styled-content style=\"fixed-case\">RSIV<\/jats:styled-content>), and genotype 3 include viruses closely related turbot reddish body iridovirus (<jats:styled-content style=\"fixed-case\">TRBIV<\/jats:styled-content>).<\/jats:p>","DOI":"10.1111\/jfd.12152","type":"journal-article","created":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T05:10:35Z","timestamp":1376716235000},"page":"609-618","source":"Crossref","is-referenced-by-count":37,"title":["Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in <scp>P<\/scp>eninsular <scp>M<\/scp>alaysia"],"prefix":"10.1111","volume":"37","author":[{"given":"K","family":"Subramaniam","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Shariff","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Universiti Putra Malaysia Selangor Malaysia"},{"name":"Institute of Bioscience Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A R","family":"Omar","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Universiti Putra Malaysia Selangor Malaysia"},{"name":"Institute of Bioscience Universiti Putra Malaysia Selangor 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