{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:53:41Z","timestamp":1788872021133,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T00:00:00Z","timestamp":1376697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japanese Society for the Promotion of Science","doi-asserted-by":"crossref","award":["21580378"],"award-info":[{"award-number":["21580378"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Serum biochemical analysis was undertaken to study the pathophysiological details of emaciation disease of the tiger puffer fish\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\n                      akifugu rubripes\n                    <\/jats:italic>\n                    (\n                    <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\n                    emminck and\n                    <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\n                    chlegel). Serum parameters were measured by biochemical analysis using automated dry chemistry and gas chromatography\/mass spectrometry (\n                    <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\n                    \/\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    ). Serum concentrations of albumin, amylase, calcium, creatinine, glucose and total protein were significantly lower in the emaciated fish when compared with those of normal fish. Regression analyses found close correlation between concentrations of total protein, albumin, amylase, glucose and progress of the disease. In contrast, serum alanine aminotransferase increased significantly in emaciated fish indicating liver function disorder. Further,\n                    <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\n                    \/\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    metabolic profiling of the puffer serum showed that the profile of the emaciated fish was distinct to that of non\u2010infected control. The serum content of amino acids including glycine, 5\u2010oxo\u2010proline and proline, and ascorbic acid, fumaric acid and glycerol increased significantly in serum in moderately emaciated fish. The serum glucose, linolenic acid and tyrosine level decreased significantly in the late phase of the disease. Our results clearly show that prolonged intestinal damage caused by myxosporean infection impairs absorption of nutrients, resulting in extreme emaciation.\n                  <\/jats:p>","DOI":"10.1111\/jfd.12154","type":"journal-article","created":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T03:24:42Z","timestamp":1376709882000},"page":"619-627","source":"Crossref","is-referenced-by-count":23,"title":["Metabolomic investigation of pathogenesis of myxosporean emaciation disease of tiger puffer fish\n                    <i>\n                      <scp>T<\/scp>\n                      akifugu rubripes\n                    <\/i>"],"prefix":"10.1111","volume":"37","author":[{"given":"H","family":"Kodama","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Immunology Course of Veterinary Science Graduate School of Agriculture and Biological Science Osaka Prefecture University Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Otani","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Immunology Course of Veterinary Science Graduate School of Agriculture and Biological Science Osaka Prefecture University Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Immunology Course of Veterinary Science Graduate School of Agriculture and Biological Science Osaka Prefecture University Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Takenaka","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Biology Course of Veterinary Science Graduate School of Agriculture and Biological Science Osaka Prefecture University Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Horitani","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Biology Course of Veterinary Science Graduate School of Agriculture and Biological Science Osaka Prefecture University Izumisano Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Togase","sequence":"additional","affiliation":[{"name":"Litanial Bioscience Laboratory Beppucho Kakogawa Hyogo 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