{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T09:35:36Z","timestamp":1758274536627,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T00:00:00Z","timestamp":1376956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"crossref","award":["AGL2009-13282-C02-01"],"award-info":[{"award-number":["AGL2009-13282-C02-01"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PROMETEO","award":["2010\/006"],"award-info":[{"award-number":["2010\/006"]}]},{"name":"ISIC","award":["2012\/003"],"award-info":[{"award-number":["2012\/003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2014,9]]},"abstract":"Abstract<\/jats:title>The enteric myxozoan parasites E<\/jats:styled-content>nteromyxum leei<\/jats:italic> (D<\/jats:styled-content>iamant, L<\/jats:styled-content>om et D<\/jats:styled-content>ykov\u00e1) and E<\/jats:styled-content>nteromyxum scophthalmi <\/jats:italic>P<\/jats:styled-content>alenzuela, R<\/jats:styled-content>edondo et \u00c1<\/jats:styled-content>lvarez\u2010Pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream (GSB<\/jats:styled-content>), S<\/jats:styled-content>parus aurata <\/jats:italic>L<\/jats:styled-content>., and to high mortality rates in turbot, P<\/jats:styled-content>setta maxima<\/jats:italic> (L<\/jats:styled-content>.). The detection of initial parasite stages in histological sections is particularly difficult, but can be simplified by means of specific antibodies. Rabbit polyclonal antibodies (pA<\/jats:styled-content>bs) were raised against E<\/jats:styled-content>.\u00a0scophthalmi<\/jats:italic> and E<\/jats:styled-content>.\u00a0leei<\/jats:italic>, and direct enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) and immunohistochemistry were used to characterize their sensitivity and specificity. Both pA<\/jats:styled-content>bs were adsorbed (apA<\/jats:styled-content>b) with non\u2010infected intestines to avoid non\u2010specific labelling of fish tissues and to improve their specificity. The highest titre obtained in ELISA<\/jats:styled-content> was 1: 32\u00a0000 for apA<\/jats:styled-content>b\u2010E<\/jats:styled-content>leei and 1:16\u00a0000 for apA<\/jats:styled-content>b\u2010E<\/jats:styled-content>scoph. Working dilutions in immunohistochemistry were 1:1000 for apA<\/jats:styled-content>b\u2010E<\/jats:styled-content>leei and 1:8000 for apA<\/jats:styled-content>b\u2010E<\/jats:styled-content>scoph. Both apA<\/jats:styled-content>bs labelled proliferative and sporogonic stages with high specificity. apA<\/jats:styled-content>b\u2010E<\/jats:styled-content>scoph was very specific, whereas apA<\/jats:styled-content>b\u2010E<\/jats:styled-content>leei cross\u2010reacted with S<\/jats:styled-content>phaerospora dicentrarchi <\/jats:italic>S<\/jats:styled-content>itj\u00e0\u2010B<\/jats:styled-content>obadilla et \u00c1<\/jats:styled-content>lvarez\u2010P<\/jats:styled-content>ellitero and S<\/jats:styled-content>phaerospora testicularis <\/jats:italic>S<\/jats:styled-content>itj\u00e0\u2010B<\/jats:styled-content>obadilla et \u00c1<\/jats:styled-content>lvarez\u2010P<\/jats:styled-content>ellitero, suggesting the presence of shared antigens. These pA<\/jats:styled-content>bs stand as new tools for antigenic characterization and the diagnosis of both E<\/jats:styled-content>nteromyxum<\/jats:italic> species.<\/jats:p>","DOI":"10.1111\/jfd.12168","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T06:36:36Z","timestamp":1376980596000},"page":"785-796","source":"Crossref","is-referenced-by-count":5,"title":["Immunohistochemical characterization of polyclonal antibodies against E<\/scp>nteromyxum leei<\/i> and E<\/scp>nteromyxum scophthalmi<\/i> (M<\/scp>yxozoa: M<\/scp>yxosporea), intestinal parasites of fish"],"prefix":"10.1111","volume":"37","author":[{"given":"I","family":"Estensoro","sequence":"first","affiliation":[{"name":"Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Cient\u00edficas (IATS\u2010CSIC) Ribera de Cabanes Castell\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"Redondo","sequence":"additional","affiliation":[{"name":"Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Cient\u00edficas (IATS\u2010CSIC) Ribera de Cabanes Castell\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"\u00c1lvarez\u2010Pellitero","sequence":"additional","affiliation":[{"name":"Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Cient\u00edficas (IATS\u2010CSIC) Ribera de Cabanes Castell\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Sitj\u00e0\u2010Bobadilla","sequence":"additional","affiliation":[{"name":"Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Cient\u00edficas (IATS\u2010CSIC) Ribera de Cabanes Castell\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-4648(95)80040-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1989.tb05534.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(1990)002<0068:CICOAM>2.3.CO;2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao048037"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0484-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2003.00653.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1988.tb00538.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(94)90276-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1996.tb00120.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2000.00227.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008525"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182005009807"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050478"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00214-8"},{"key":"e_1_2_6_17_1","first-page":"241","article-title":"Comparative studies on carbohydrates of several myxosporean parasites of fish using lectin histochemical methods","volume":"46","author":"Mu\u00f1oz P.","year":"1999","journal-title":"Folia Parasitologica"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao040117"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.4269\/ajtmh.1995.52.89","article-title":"Use of cross\u2010reactive antigens of the microsporidian Glugea atherinae for the possible detection of Enterocytozoon bieneusi by western\u2010blot","volume":"52","author":"Ombrouck C.","year":"1995","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_6_20_1","first-page":"639","volume-title":"Biotechnologies for Quality","author":"Palenzuela O.","year":"2004"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2008.05.012"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2011.01310.x"},{"key":"e_1_2_6_23_1","unstructured":"RedondoM.J.(2005)Estudios sobre el ciclo vital y transmisi\u00f3n de Enteromyxum scophthalmi (Myxozoa) par\u00e1sito ent\u00e9rico del rodaballo. 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