{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T06:23:03Z","timestamp":1769840583593,"version":"3.49.0"},"reference-count":68,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,9,24]],"date-time":"2013-09-24T00:00:00Z","timestamp":1379980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Foreign Affairs"},{"name":"International Trade Canada"},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infectious spleen and kidney necrosis virus (ISKNV), family <jats:italic>Iridoviridae<\/jats:italic>, genus <jats:italic>Megalocytivirus,<\/jats:italic> may cause high mortality rates such as those seen in mandarin fish, <jats:italic>Siniperca chuatsi<\/jats:italic>. ISKNV has attracted much attention due to the possible environmental threat and economic losses it poses on both cultured and wild populations. We have investigated the pathogenicity of ISKNV\u2010like agent <jats:italic>Megalocytivirus<\/jats:italic>, isolated from infected pearl gourami, in golden mandarin fish, <jats:italic>Siniperca scherzeri<\/jats:italic> \u2013 a member of the <jats:italic>Percichthyidae<\/jats:italic> family \u2013 and in another <jats:italic>Percichthyidae<\/jats:italic> species, <jats:italic>S.\u00a0chuatsi<\/jats:italic>. Fish were challenged with four different doses of ISKNV\u2010like agent <jats:italic>Megalocytivirus<\/jats:italic> (1, 10, 100 or 1000\u00a0\u03bcg per fish) over a 30\u2010day period, and cumulative fish mortalities were calculated for each group. No significant mortality was observed for fish challenged with the lowest dose (1\u00a0\u03bcg per fish) relative to a control group. However, all other challenged groups showed 100% mortality over a 30\u2010day period in proportion to the challenge dose. Quantitative real\u2010time PCR was performed to measure <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression levels for six immune\u2010related genes in golden mandarin fish following ISKNV\u2010like agent challenge. <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression levels for IRF1, Mx, viperin and interleukin 8 significantly increased, while <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels for IRF2 and IRF7 remained constant or declined during the challenge period.<\/jats:p>","DOI":"10.1111\/jfd.12182","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:03:06Z","timestamp":1381528986000},"page":"1041-1054","source":"Crossref","is-referenced-by-count":12,"title":["Disease resistance and immune\u2010relevant gene expression in golden mandarin fish, <i>Siniperca scherzeri<\/i> Steindachner, infected with infectious spleen and kidney necrosis virus\u2010like agent"],"prefix":"10.1111","volume":"37","author":[{"given":"G W","family":"Shin","sequence":"first","affiliation":[{"name":"Fundamental Research Department National Fisheries Research and Development Institute Busan Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S L","family":"White","sequence":"additional","affiliation":[{"name":"Department of Fisheries and Oceans Centre for Aquaculture and Environmental Research West Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H U","family":"Dahms","sequence":"additional","affiliation":[{"name":"Department of Life Science College of Natural Science Sangmyung University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H D","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Aquatic Life Medicine Pukyong National University Busan Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J H","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Fisheries and Oceans Centre for Aquaculture and Environmental Research West Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2004.03.009"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/107999002753452665"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(2000)012<0301:VCOTBS>2.0.CO;2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.11.022"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2003.00494.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2010.01.039"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02021.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-4648(03)00011-1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.01.021"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.04.015"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12562-010-0275-x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.04.004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2011.03.008"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2010.0127"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2012.07.012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.12.002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.2.1500"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2000.00213.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(01)00639-1"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1958-4"},{"key":"e_1_2_6_22_1","unstructured":"HuckstorfV.(2012)Siniperca scherzeri. In: IUCN 2012. IUCN Red List of Threatened Species. In: Africa Research Bulletin: Political Social and Cultural Series. IUCN."},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.27.19"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao01961"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao01879"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.04.061"},{"key":"e_1_2_6_27_1","volume-title":"Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses","author":"King A.M.","year":"2011"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-305X(01)00092-1"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-305X(99)00060-9"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00600-X"},{"key":"e_1_2_6_31_1","first-page":"57","article-title":"Sexual maturation and feeding habit of Korean mandarin fish, Siniperca scherzeri (Perciformes, Centropomidae) in the Seomjin river and Imjin river, Korea","volume":"26","author":"Lee W.O.","year":"2012","journal-title":"Korean Journal of Environmental Ecology"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.05.021"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2011.06.006"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(99)00262-0"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.11.015"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.1998.00082.x"},{"key":"e_1_2_6_38_1","first-page":"145","article-title":"Morphological and microsatellite analysis of Siniperca chautsi \u2640\u00d7 Siniperca scherzeri \u2642 hybrid with their parents","volume":"19","author":"Mi G.Q.","year":"2010","journal-title":"Journal of Shanghai Ocean University"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199902)29:02<375::AID-IMMU375>3.0.CO;2-6"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.3.1842-1852.2005"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.04.016"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13089534"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2001.0991"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.36.38"},{"key":"e_1_2_6_46_1","first-page":"898","article-title":"A comparative study of the nutrients and amino acid composition of the muscle of Siniperca chuatsi and Siniperca scherzeri","volume":"27","author":"Su S.Q.","year":"2005","journal-title":"Journal of Southwest Agricultural University"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2004.04.013"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400525101"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2004.12.001"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2006.10.009"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.10.6005"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090400"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.07.002"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.03.012"},{"key":"e_1_2_6_55_1","first-page":"589","article-title":"Genetic variation of the mitochondrial DNA Cyt b among six populations of Siniperca scherzeri in China","volume":"27","author":"Wang W.W.","year":"2006","journal-title":"Zoological Research"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-006-0870-4"},{"key":"e_1_2_6_57_1","doi-asserted-by":"crossref","first-page":"25589","DOI":"10.1016\/S0021-9258(19)74081-2","article-title":"Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon\u2010inducible genes","volume":"267","author":"Weisz A.","year":"1992","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2002.00419.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(08)60076-7"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.06.009"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.07.003"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.12.026"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00851-10"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.07.003"},{"key":"e_1_2_6_65_1","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1007\/s00251-002-0525-x","article-title":"Molecular cloning of the pufferfish (Takifugu rubripes) Mx gene and functional characterization of its promoter","volume":"54","author":"Yap W.H.","year":"2003","journal-title":"Immunogenetics"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-004-0658-1"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.03.137"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2009.02328.x"},{"key":"e_1_2_6_69_1","first-page":"113","article-title":"On the classification and distribution of the Sinipercinae fishes (Family Serranidae)","volume":"9","author":"Zhou C.","year":"1988","journal-title":"Zoological Research"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12182","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12182","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:03:01Z","timestamp":1696168981000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,24]]},"references-count":68,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/jfd.12182"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12182","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,24]]}}}