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The genome of <jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content> codes for five proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010dependent <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> polymerase (L). <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010mediated interference (<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i) by small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) is a powerful tool to inhibit gene transcription and is used to study genes important for viral replication. In previous studies regarding another member of <jats:italic><jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>habdoviridae<\/jats:italic>, si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> inhibition of the rabies virus nucleoprotein gene provided <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> protection against rabies. In this study, synthetic si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> molecules were designed to target <jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content>\u2010N and <jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content>\u2010P transcripts to inhibit <jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content> replication and were tested in an <jats:italic>epithelioma papulosum cyprini<\/jats:italic> (<jats:styled-content style=\"fixed-case\">EPC<\/jats:styled-content>) cell line. Inhibition of gene transcription was measured by real\u2010time quantitative reverse\u2010transcription <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>). The efficacy of using si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> for inhibition of viral replication was analysed by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> measurement of a reporter gene (glycoprotein) expression and by virus endpoint titration. Inhibition of nucleoprotein and phosphoprotein gene expression by si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> reduced <jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content> replication. However, use of tandem si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s that target phosphoprotein and nucleoprotein worked best at reducing <jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content> replication.<\/jats:p>","DOI":"10.1111\/jfd.12227","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T11:50:51Z","timestamp":1390823451000},"page":"197-207","source":"Crossref","is-referenced-by-count":51,"title":["Inhibition of spring viraemia of carp virus replication in an <i><scp>E<\/scp>pithelioma papulosum cyprini<\/i> cell line by <scp>RNA<\/scp>i"],"prefix":"10.1111","volume":"38","author":[{"given":"M","family":"Gotesman","sequence":"first","affiliation":[{"name":"Clinical Division of Fish Medicine University of Veterinary Medicine Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Soliman","sequence":"additional","affiliation":[{"name":"Clinical Division of Fish 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