{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:35:05Z","timestamp":1764603305215,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,10,27]],"date-time":"2014-10-27T00:00:00Z","timestamp":1414368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["NRF-2013R1A1A 2005217"],"award-info":[{"award-number":["NRF-2013R1A1A 2005217"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Pukyong National University Research Fund","award":["(CD20131344)"],"award-info":[{"award-number":["(CD20131344)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptococcus parauberis<\/jats:italic> is an increasing threat to aquaculture of olive flounder, <jats:italic>Paralichthys olivaceus<\/jats:italic> Temminck &amp; Schlegel, in South Korea. We developed a real\u2010time polymerase chain reaction (PCR) method using the TaqMan probe assay to detect and quantify <jats:italic>S.\u00a0parauberis<\/jats:italic> by targeting the <jats:italic>gyrB<\/jats:italic> gene sequences, which are effective for molecular analysis of the genus <jats:italic>Streptococcus<\/jats:italic>. Our real\u2010time PCR assay is capable of detecting 10\u00a0fg of genomic DNA per reaction. The intra\u2010 and interassay coefficient of variation (CV) values ranged from 0.42\u20131.95%, demonstrating that the assay has good reproducibility. There was not any cross\u2010reactivity to <jats:italic>Streptococcus iniae<\/jats:italic> or to other streptococcal\/lactococcal fish pathogens, such as <jats:italic>S.\u00a0agalactiae and Lactococcus garvieae<\/jats:italic>, indicating that the assay is highly specific to <jats:italic>S.\u00a0parauberis<\/jats:italic>. The results of the real\u2010time PCR assay corresponded well to those of conventional culture assays for <jats:italic>S.\u00a0parauberis<\/jats:italic> from inoculated tissue homogenates (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.957; <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). Hence, this sensitive and specific real\u2010time PCR is a valuable tool for diagnostic quantitation of <jats:italic>S.\u00a0parauberis<\/jats:italic> in clinical samples.<\/jats:p>","DOI":"10.1111\/jfd.12322","type":"journal-article","created":{"date-parts":[[2014,10,27]],"date-time":"2014-10-27T08:27:26Z","timestamp":1414398446000},"page":"31-39","source":"Crossref","is-referenced-by-count":21,"title":["Development of real\u2010time PCR for detection and quantitation of <i>Streptococcus parauberis<\/i>"],"prefix":"10.1111","volume":"39","author":[{"given":"T L","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Aquatic Life Medicine College of Fisheries Science Pukyong National University Busan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y J","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Aquatic Life Medicine College of Fisheries Science Pukyong National University Busan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u2010H","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Aquatic Life Medicine College of Fisheries Science Pukyong National University Busan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Austin","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Pathfoot Building University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00753.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2006.7.1.53"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-4-487"},{"key":"e_1_2_7_5_1","first-page":"209","article-title":"Isolation of Streptococcus parauberis from starry flounder, Platichthys stellatus Pallas","volume":"21","author":"Cho M.Y.","year":"2008","journal-title":"J Fish Pathol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2779-2785.2002"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00343-012-1244-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.30.1.79"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2005.12.003"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02639-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.44.33"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1128\/JCM.38.1.301-308.2000","article-title":"Differentiation of phylogenetically related slowly growing mycobacteria by their gyrB sequences","volume":"38","author":"Kasai H.","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-406"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02920-12"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-172"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-72.6.1310"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.5.3183-3187.2004"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01491.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00182-11"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080395"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.10.002"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00780-13"},{"key":"e_1_2_7_23_1","unstructured":"StaroscikA.(2004)Calculator for determining the number of copies of a template. 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