{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:44:49Z","timestamp":1790095489841,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T00:00:00Z","timestamp":1435622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Feeding aquatic animals with bacterial encapsulated heat\u2010shock proteins (Hsps) is potentially a new method to combat vibriosis, an important disease affecting aquatic animals used in aquaculture. Food pellets comprised of shrimp and containing <jats:italic>Escherichia coli<\/jats:italic> overexpressing either DnaK\u2010DnaJ\u2010GrpE, the prokaryotic equivalents of Hsp70\u2010Hsp40\u2010Hsp20, or only DnaK were fed to juveniles of the white leg shrimp <jats:italic>Penaeus vannamei<\/jats:italic>, and protection against pathogenic <jats:italic>Vibrio harveyi<\/jats:italic> was determined. Maintaining pellets at different temperatures for varying lengths of time reduced the number of live adhering <jats:italic>E.\u00a0coli<\/jats:italic>, as did contact with sea water, demonstrating that storage and immersion adversely affected bacterial survival and attachment to pellets. Feeding <jats:italic>P.\u00a0vannamei<\/jats:italic> with <jats:italic>E.\u00a0coli<\/jats:italic> did not compromise their survival, indicating that the bacteria were not pathogenic to shrimp. Feeding <jats:italic>P.\u00a0vannamei<\/jats:italic> with pellets containing bacteria overproducing DnaK (approximately 60\u00a0cells\u00a0g<jats:sup>\u22121<\/jats:sup> pellets) boosted <jats:italic>P.\u00a0vannamei<\/jats:italic> survival twofold against <jats:italic>V.\u00a0harveyi<\/jats:italic>, suggesting that DnaK plays a role in <jats:italic>Vibrio<\/jats:italic> tolerance. Pellets containing DnaK were effective in providing protection to <jats:italic>P.\u00a0vannamei<\/jats:italic> for up to 2\u00a0weeks before loss of viability and that DnaK encapsulated by these bacteria enhanced shrimp resistance against <jats:italic>Vibrio<\/jats:italic> infection.<\/jats:p>","DOI":"10.1111\/jfd.12390","type":"journal-article","created":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T09:45:54Z","timestamp":1435657554000},"page":"577-584","source":"Crossref","is-referenced-by-count":14,"title":["Ingestion of food pellets containing <i>Escherichia coli<\/i> overexpressing the heat\u2010shock protein DnaK protects <i>Penaeus vannamei<\/i> (Boone) against <i>Vibrio harveyi<\/i> (Baumann) infection"],"prefix":"10.1111","volume":"39","author":[{"given":"S","family":"Sinnasamy","sequence":"first","affiliation":[{"name":"Institute of Marine Biotechnology University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Mat Noordin","sequence":"additional","affiliation":[{"name":"School of Fisheries and Aquaculture Sciences University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T H","family":"MacRae","sequence":"additional","affiliation":[{"name":"Department of Biology Dalhousie University Halifax NS Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Ikhwanuddin bin Abdullah","sequence":"additional","affiliation":[{"name":"School of Fisheries and Aquaculture Sciences University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Bossier","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture &amp; Artemia Reference Center Faculty of Bioscience Engineering Ghent University Gent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M E","family":"bin Abdul Wahid","sequence":"additional","affiliation":[{"name":"Institute of Marine Biotechnology University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia"},{"name":"School of Fisheries and Aquaculture Sciences University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Noriaki","sequence":"additional","affiliation":[{"name":"Agrobest Malaysia Sdn. Bhd Pekan Pahang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y Y","family":"Sung","sequence":"additional","affiliation":[{"name":"Institute of Marine Biotechnology University Malaysia Terengganu (UMT) Kuala Terengganu Malaysia"},{"name":"School of Fisheries and Aquaculture Sciences University Malaysia Terengganu (UMT) Kuala Terengganu 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