{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T15:54:28Z","timestamp":1720626868790},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,6,3]],"date-time":"2016-06-03T00:00:00Z","timestamp":1464912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["PROMETEO 2010\/006","ISIC 2012\/003"],"award-info":[{"award-number":["PROMETEO 2010\/006","ISIC 2012\/003"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Systemic amoebiasis of sole is caused by <jats:italic>Endolimax piscium<\/jats:italic>, a cryptic parasitic archamoeba whose epidemiology and pathogeny are yet unknown. To establish reliable detection methods for this parasite, a battery of molecular diagnostic tools (<jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>) were developed and evaluated with a panel of clinical samples from symptomatic diseased fish and from apparently normal animals of different stocks. As there is neither enough background information on the epidemiology of the disease nor a validated reference method, comparison of tests used a composite reference method approach. The <jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content> technique was the most specific and sensitive in intestine samples and particularly useful as a reference confirmatory method, while the best method in muscle samples was <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Application of the tests to asymptomatic fish demonstrated presence of parasites in a large proportion (&gt;25%) of their intestines, suggesting that this is the point of entry of the amoebae and the initial stage in the development of the disease. The triggering factors that facilitate the breaching of the intestinal barrier by <jats:italic>E.\u00a0piscium<\/jats:italic>, causing granulomatous lesions in other organs and systemic spreading, are not completely understood but our results point to the connective tissue as a preferential target for parasite development and migration.<\/jats:p>","DOI":"10.1111\/jfd.12480","type":"journal-article","created":{"date-parts":[[2016,6,4]],"date-time":"2016-06-04T04:46:40Z","timestamp":1465015600000},"page":"1433-1443","source":"Crossref","is-referenced-by-count":4,"title":["Development of different diagnostic techniques for <i>Endolimax piscium<\/i> (archamoebae) and their applicability in <i>Solea senegalensis<\/i> clinical samples"],"prefix":"10.1111","volume":"39","author":[{"given":"M","family":"Constenla","sequence":"first","affiliation":[{"name":"Departament de Biologia Animal de Biologia Vegetal i d'Ecologia and Servei de Diagn\u00f2stic Patol\u00f2gic en Peixos XRAq (Generalitat de Catalunya) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Padr\u00f3s","sequence":"additional","affiliation":[{"name":"Departament de Biologia Animal de Biologia Vegetal i d'Ecologia and Servei de Diagn\u00f2stic Patol\u00f2gic en Peixos XRAq (Generalitat de Catalunya) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"del Pozo","sequence":"additional","affiliation":[{"name":"Instituto de Acuicultura de Torre de la Sal (IATS\u2010CSIC) Castell\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Palenzuela","sequence":"additional","affiliation":[{"name":"Instituto de Acuicultura de Torre de la Sal (IATS\u2010CSIC) Castell\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050662"},{"key":"e_1_2_6_3_1","unstructured":"deBlasI. 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