{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T06:43:38Z","timestamp":1768632218836,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report the first description of a new <jats:italic>Rhabdoviridae<\/jats:italic> tentatively named eelpout rhabdovirus (Ep<jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content> genus <jats:italic>Perhabdovirus<\/jats:italic>). This virus was associated with mass mortalities in eelpout (<jats:italic>Zoarces viviparous,<\/jats:italic> Linnaeus) along the Swedish Baltic Sea coast line in 2014. Diseased fish showed signs of central nervous system infection, and brain lesions were confirmed by histology. A cytopathogenic effect was observed in cell culture, but <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>s for the epizootic piscine viral haemorrhagic septicaemia virus (<jats:styled-content style=\"fixed-case\">VHSV<\/jats:styled-content>), infectious pancreas necrosis virus (<jats:styled-content style=\"fixed-case\">IPNV<\/jats:styled-content>), infectious haematopoietic necrosis virus (<jats:styled-content style=\"fixed-case\">IHNV<\/jats:styled-content>) and spring viraemia of carp virus (<jats:styled-content style=\"fixed-case\">SVCV<\/jats:styled-content>) were negative. Further investigations by chloroform inactivation, indirect fluorescence antibody test and electron microscopy indicated the presence of a rhabdovirus. By deep sequencing of original tissue suspension and infected cell culture supernatant, the full viral genome was assembled and we confirmed the presence of a rhabdovirus with 59.5% nucleotide similarity to the closest relative Siniperca chuatsi rhabdovirus. The full\u2010genome sequence of this new virus, eelpout rhabdovirus (Ep<jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content>), has been deposited in GenBank under accession number <jats:styled-content style=\"fixed-case\">KR<\/jats:styled-content>612230. An <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> based on the L\u2010gene sequence confirmed the presence of Ep<jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content> in sick\/dead eelpout, but the virus was not found in control fish. Additional investigations to characterize the pathogenicity of Ep<jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content> are planned.<\/jats:p>","DOI":"10.1111\/jfd.12506","type":"journal-article","created":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T08:43:18Z","timestamp":1468572198000},"page":"219-229","source":"Crossref","is-referenced-by-count":11,"title":["Emergence of a new rhabdovirus associated with mass mortalities in eelpout (<i>Zoarces viviparous<\/i>) in the Baltic Sea"],"prefix":"10.1111","volume":"40","author":[{"given":"C","family":"Ax\u00e9n","sequence":"first","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Hakhverdyan","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T S","family":"Boutrup","sequence":"additional","affiliation":[{"name":"EU reference laboratory for fish diseases Technical University of Denmark Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Blomkvist","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Ljunghager","sequence":"additional","affiliation":[{"name":"Swedish Agency for Marine and Water Management Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Alfjorden","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c5","family":"Hagstr\u00f6m","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N J","family":"Olesen","sequence":"additional","affiliation":[{"name":"EU reference laboratory for fish diseases Technical University of Denmark Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Juremalm","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Leijon","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u2010F","family":"Valarcher","sequence":"additional","affiliation":[{"name":"National Veterinary Institute Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.211424698"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504666102"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","unstructured":"Anonymous(2001).2001\/83\/EC. 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