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The international trade of juvenile fish for food and ornamental aquaculture has aided the spread of these viruses, which have spread to Europe and Australia and other regions. Australian freshwater fishes were examined for susceptibility to infection with the exotic megalocytivirus, dwarf gourami iridovirus (<jats:styled-content style=\"fixed-case\">DGIV<\/jats:styled-content>), which belongs to a group with the type species, <jats:italic>Infectious spleen and kidney necrosis virus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content>). Fish were held at 23\u00a0\u00b1\u00a01\u00a0\u00b0C and challenged by intraperitoneal (<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>) injection or by cohabitation with Murray cod, <jats:italic>Maccullochella peelii<\/jats:italic> (Mitchell) infected with <jats:styled-content style=\"fixed-case\">DGIV<\/jats:styled-content>. A species was deemed to be susceptible to <jats:styled-content style=\"fixed-case\">DGIV<\/jats:styled-content> based on evidence of viral replication, as determined by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>, and megalocytic inclusion bodies observed histologically. Horizontal transmission occurred between infected Murray cod and golden perch, <jats:italic>Macquaria ambigua<\/jats:italic> (Richardson), Macquarie perch, <jats:italic>Macquaria australasica<\/jats:italic> (Cuvier) and Murray cod. This indicated that <jats:styled-content style=\"fixed-case\">DGIV<\/jats:styled-content> shed from infected fish held at 23\u00a0\u00b0C can survive in fresh water and subsequently infect these na\u00efve fish. Further, <jats:styled-content style=\"fixed-case\">DGIV<\/jats:styled-content> administered <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> was highly pathogenic to golden perch, Macquarie perch and Murray cod. Compared to these species, the susceptibility of southern pygmy perch, <jats:italic>Nannoperca australis<\/jats:italic> (Gunther) was lower. Freshwater catfish (dewfish), <jats:italic>Tandanus tandanus<\/jats:italic> (Mitchell), were not susceptible under the experimental conditions based on the absence of clinical disease, mortality and virus replication. This study showed the potential risks associated with na\u00efve and <jats:styled-content style=\"fixed-case\">DGIV<\/jats:styled-content>\u2010infected fish sharing a common water source.<\/jats:p>","DOI":"10.1111\/jfd.12510","type":"journal-article","created":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T10:06:18Z","timestamp":1466762778000},"page":"293-310","source":"Crossref","is-referenced-by-count":30,"title":["Susceptibility of a number of Australian freshwater fishes to dwarf gourami iridovirus (<i>Infectious spleen and kidney necrosis virus<\/i>)"],"prefix":"10.1111","volume":"40","author":[{"given":"A E","family":"Rimmer","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science School of Life and Environmental Sciences The University of Sydney Camden NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R J","family":"Whittington","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science School of Life and Environmental Sciences The University of Sydney Camden NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Tweedie","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science School of Life and Environmental Sciences The University of Sydney Camden NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Becker","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science School of Life and Environmental Sciences The University of Sydney Camden NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1993.tb15144.x"},{"key":"e_1_2_7_4_1","unstructured":"Australian Department of Agriculture and Water Resources 2015"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/08997659.2012.747451"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao01845"},{"key":"e_1_2_7_7_1","volume-title":"An Introduction to Survival Analysis Using Stata","author":"Cleves M.","year":"2008"},{"key":"e_1_2_7_8_1","volume-title":"Veterinary Epidemiologic Research","author":"Dohoo I.","year":"2003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.04.033"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.12.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2010.01234.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(1998)010<0107:ROTHPA>2.0.CO;2"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1993.tb00850.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao01961"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/v4040521"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/08997659.2011.545697"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2003.tb12512.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1989.tb00318.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/00288330.2004.9517255"},{"key":"e_1_2_7_20_1","volume-title":"Fishes of the Murray\u2010Darling Basin: An Introductory Guide","author":"Lintermans M","year":"2007"},{"key":"e_1_2_7_21_1","first-page":"14","article-title":"GeneDoc: analysis and visualization of genetic variation","volume":"4","author":"Nicholas K.B.","year":"1997","journal-title":"Embnew. 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