{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:36:49Z","timestamp":1787020609739,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,7,2]],"date-time":"2016-07-02T00:00:00Z","timestamp":1467417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007359","name":"Fondazione Banco di Sardegna","doi-asserted-by":"publisher","award":["RF 2014\u20101115"],"award-info":[{"award-number":["RF 2014\u20101115"]}],"id":[{"id":"10.13039\/100007359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Mycobacterium marinum<\/jats:italic> is a slow\u2010growing non\u2010tuberculous mycobacterium, and it is considered the most common aetiologic agent of mycobacteriosis in wild and cultured fish. The diagnosis is principally made by histology when positive Ziehl\u2013Neelsen stain granulomas are detected. The aim of this study was to investigate the occurrence of mycobacteriosis in extensively cultured Mugilidae of two lagoons (Cabras and San Teodoro) from Sardinia by the use of histology, microbiology, PCR and DNA sequencing. Nine of 106 mullets examined were affected by mycobacteriosis, and the spleen was the most affected organ. The histology detected higher rate (100%) of infection in spleen than the culture and PCR (75% and 62.5%, respectively). The sequencing of hsp65 gene identified <jats:italic>M.\u00a0marinum<\/jats:italic> as the primary cause of mycobacteriosis in the mullets examined. Mullets affected by mycobacteriosis were mainly fished in the San Teodoro lagoon characterized by critical environmental conditions. Histology remains the most common method in detecting fish affected by mycobacteriosis, and PCR\u2010based methods are essential for species identification. Our finding are worthy of attention because mycobacteriosis caused by <jats:italic>M.\u00a0marinum<\/jats:italic> in reared mullets was evidenced for the first time in Sardinia, suggesting that this disease may be underestimated also in other cultured fish species.<\/jats:p>","DOI":"10.1111\/jfd.12515","type":"journal-article","created":{"date-parts":[[2016,7,2]],"date-time":"2016-07-02T06:22:12Z","timestamp":1467440532000},"page":"327-337","source":"Crossref","is-referenced-by-count":19,"title":["Mycobacteriosis caused by <i>Mycobacterium marinum<\/i> in reared mullets: first evidence from Sardinia (Italy)"],"prefix":"10.1111","volume":"40","author":[{"given":"E","family":"Antuofermo","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Pais","sequence":"additional","affiliation":[{"name":"Laboratory of Aquaculture and Aquatic Resources Management Section\u00a0of Animal Sciences Department of Agriculture University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Polinas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Cubeddu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Righetti","sequence":"additional","affiliation":[{"name":"Fish Disease Laboratory State Veterinary Institute of Piedmont, Liguria and Aosta Valley Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Sanna","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Sassari Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Prearo","sequence":"additional","affiliation":[{"name":"Fish Disease Laboratory State Veterinary Institute of Piedmont, Liguria and Aosta Valley Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0218339014500028"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-18"},{"key":"e_1_2_8_4_1","first-page":"666","article-title":"Diagnosis and management of atypical Mycobacterium spp. infections in established laboratory zebrafish (Brachydanio rerio) facilities","volume":"50","author":"Astrofsky K.M.","year":"2000","journal-title":"Comparative Medicine"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2006.00729.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2010.01167.x"},{"key":"e_1_2_8_7_1","first-page":"45","article-title":"Mycobacterium marinum produces long\u2010term chronic infections in medaka: a new animal model for studying human tuberculosis","volume":"145","author":"Broussard G.W.","year":"2006","journal-title":"Comparative Biochemistry and Physiology Part C"},{"key":"e_1_2_8_8_1","volume-title":"Gli Stagni Della Sardegna. 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