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Changes in the antigenic composition of this parasite occur during its developmental stages. In this study, we evaluated humoral parameters in serum and transcriptional immune responses of yellowtail naturally infected with <jats:italic>N.\u00a0melleni<\/jats:italic>. In addition, <jats:italic>in\u00a0vitro<\/jats:italic> assays were performed to study the stimulatory effects of antigens from larvae and adults on spleen leucocytes from non\u2010infected fish at 6 and 24\u00a0h post\u2010stimulation. The results showed enhanced total protein, myeloperoxidase and antiprotease activities in <jats:italic>N.\u00a0melleni<\/jats:italic>\u2010infected fish compared with non\u2010infected ones. The induction of Toll\u2010like receptors (TLRs) and pro\u2010inflammatory cytokines in spleen leucocytes during natural infection with <jats:italic>N.\u00a0melleni<\/jats:italic> suggests that these immune\u2010related genes play an important role in the initiation of the immune defence mechanism for controlling parasite infection. Interestingly, the magnitude of <jats:italic>in\u00a0vitro<\/jats:italic> responses of spleen leucocytes was dependent on the parasitic stage. An important stimulation of gene expression by adult antigens on spleen leucocytes was observed. Differential expression patterns of TLRs and target cytokines in yellowtail leucocytes in both <jats:italic>in\u00a0vivo<\/jats:italic> and <jats:italic>in\u00a0vitro<\/jats:italic> studies suggest that the quality of yellowtail immune response is conditioned by <jats:italic>N.\u00a0melleni<\/jats:italic> development.<\/jats:p>","DOI":"10.1111\/jfd.12620","type":"journal-article","created":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T11:19:23Z","timestamp":1492600763000},"page":"1497-1509","source":"Crossref","is-referenced-by-count":19,"title":["<i>In vivo<\/i> and <i>in\u00a0vitro<\/i> studies using larval and adult antigens from <i>Neobenedenia melleni<\/i> on immune response in yellowtail (<i>Seriola lalandi<\/i>)"],"prefix":"10.1111","volume":"40","author":[{"given":"M","family":"Reyes\u2010Becerril","sequence":"first","affiliation":[{"name":"Immunology and Vaccinology Group Centro de Investigaciones Biol\u00f3gicas del Noroeste (CIBNOR) La Paz Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Alamillo","sequence":"additional","affiliation":[{"name":"Immunology and Vaccinology Group Centro de Investigaciones Biol\u00f3gicas del Noroeste (CIBNOR) La Paz Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Trasvi\u00f1a","sequence":"additional","affiliation":[{"name":"Immunology and Vaccinology Group Centro de Investigaciones Biol\u00f3gicas del Noroeste (CIBNOR) La Paz Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Hirono","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Kondo","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Jirapongpairoj","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Ascencio\u2010Valle","sequence":"additional","affiliation":[{"name":"Immunology and Vaccinology Group Centro de Investigaciones Biol\u00f3gicas del Noroeste (CIBNOR) La Paz Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7965-1679","authenticated-orcid":false,"given":"C","family":"Angulo","sequence":"additional","affiliation":[{"name":"Immunology and Vaccinology Group Centro de Investigaciones Biol\u00f3gicas del Noroeste (CIBNOR) La Paz Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,19]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"AbbasA.K. 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