{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:20:40Z","timestamp":1791418840133,"version":"4.3.3"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T00:00:00Z","timestamp":1494460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["237315\/E40"],"award-info":[{"award-number":["237315\/E40"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This work reports the effect of two <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccines against salmonid alphavirus 3 (<jats:styled-content style=\"fixed-case\">SAV<\/jats:styled-content>3) in Atlantic salmon. Presmolts were vaccinated by intramuscular injection of plasmids encoding the <jats:styled-content style=\"fixed-case\">SAV<\/jats:styled-content>3 structural polyprotein C\u2010E3\u2010E2\u20106K\u2010E2 (<jats:styled-content style=\"fixed-case\">pCSP<\/jats:styled-content>), E2 only (<jats:styled-content style=\"fixed-case\">pE<\/jats:styled-content>2), or plasmid without insert (pc<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>3.3). E2 is expressed at the surface of cells transfected with <jats:styled-content style=\"fixed-case\">pCSP<\/jats:styled-content> and internally in cells transfected with <jats:styled-content style=\"fixed-case\">pE<\/jats:styled-content>2. A commercial vaccine based on inactivated <jats:styled-content style=\"fixed-case\">SAV<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">NCPD<\/jats:styled-content>) was used for comparison. At 10\u00a0weeks post\u2010vaccination, only fish vaccinated with <jats:styled-content style=\"fixed-case\">pCSP<\/jats:styled-content> showed antibody against E2 and virus\u2010neutralizing activity. Vaccinated fish were infected with <jats:styled-content style=\"fixed-case\">SAV<\/jats:styled-content>3 to determine protection by virus quantitation in serum after 7\u00a0days and scoring of pathological changes after 21\u00a0days. Fish vaccinated with both <jats:styled-content style=\"fixed-case\">pCSP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NCPD<\/jats:styled-content> vaccines showed significant virus reduction in serum, while fish vaccinated with <jats:styled-content style=\"fixed-case\">pE<\/jats:styled-content>2 did not. All fish vaccinated with pc<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>3.3 and <jats:styled-content style=\"fixed-case\">pE<\/jats:styled-content>2 showed pathological changes in organs typical of <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>, 60% of fish vaccinated with <jats:styled-content style=\"fixed-case\">NCPD<\/jats:styled-content> showed <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> pathology, while fish vaccinated with <jats:styled-content style=\"fixed-case\">pCSP<\/jats:styled-content> did not show <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content> pathology. Taken together, <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccination with <jats:styled-content style=\"fixed-case\">pCSP<\/jats:styled-content> provided strong protection for salmon against <jats:styled-content style=\"fixed-case\">SAV<\/jats:styled-content>3 infection, which in part may be due to production of virus\u2010neutralizing antibodies.<\/jats:p>","DOI":"10.1111\/jfd.12644","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T16:06:38Z","timestamp":1494518798000},"page":"1775-1781","source":"Crossref","is-referenced-by-count":39,"title":["Protective effect and antibody response of <scp>DNA<\/scp> vaccine against salmonid alphavirus 3 (<scp>SAV<\/scp>3) in Atlantic salmon"],"prefix":"10.1111","volume":"40","author":[{"given":"C J","family":"Chang","sequence":"first","affiliation":[{"name":"Norwegian College of Fishery Science UiT The Arctic University of Norway Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Gu","sequence":"additional","affiliation":[{"name":"Norwegian Veterinary Institute Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0481-9868","authenticated-orcid":false,"given":"B","family":"Robertsen","sequence":"additional","affiliation":[{"name":"Norwegian College of Fishery Science UiT The Arctic University of Norway Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02529"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2016.08.020"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.05.059"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.03.093"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.119.12.297"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1986.tb00987.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.00944.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2010.01234.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.09.026"},{"key":"e_1_2_7_11_1","volume-title":"The Fish Health Report 2015","author":"Hjeltnes B.","year":"2016"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2009.06.012"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.04.074"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.24.1.1565"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2007.00848.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025816"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-44-7"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao022025"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao012017"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.135.10.234"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.58.3.491-562.1994"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.05.010"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.03.081"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12644","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T01:14:05Z","timestamp":1694999645000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,11]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/jfd.12644"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12644","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,11]]}}}