{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:00:35Z","timestamp":1772823635706,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006488","name":"Institut National de la Recherche Agronomique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2018,1]]},"abstract":"Abstract<\/jats:title>Infectious haematopoietic necrosis virus (IHNV<\/jats:styled-content>) and spring viraemia of carp virus (SVCV<\/jats:styled-content>) are both rhabdoviruses of fish, listed as notifiable disease agents by the World Organization for Animal Health. Recombinant rhabdoviruses with heterologous gene substitutions have been engineered to study genetic determinants and assess the potential of these recombinant viruses for vaccine development. A recombinant IHNV<\/jats:styled-content> (rIHNV<\/jats:styled-content>), containing the full\u2010length genome of a European IHNV<\/jats:styled-content> strain, was modified by deleting the glycoprotein (G) gene and replacing it with a European SVCV<\/jats:styled-content> G\u2010gene to make the rIHNV<\/jats:styled-content>\u2010Gsvcv. The chimeric rIHNV<\/jats:styled-content>\u2010Gsvcv level of virulence in rainbow trout, common carp and koi was assessed, and its ability to induce a protective immune response in surviving koi against wild\u2010type SVCV<\/jats:styled-content> infection was tested. The rIHNV<\/jats:styled-content>\u2010Gsvcv infection of trout led to high mortality, ranging from 78% to 92.5%, after immersion. In contrast, no deaths occurred in juvenile common carp after infection with rIHNV<\/jats:styled-content>\u2010Gsvcv by either immersion or intraperitoneal (IP<\/jats:styled-content>) injection. Similarly, koi infected with rIHNV<\/jats:styled-content>\u2010Gsvcv via IP<\/jats:styled-content> injection had little to no mortality (\u22649%). Koi that survived initial infection with a high dose of recombinant virus rIHNV<\/jats:styled-content>\u2010Gsvcv were protected against a virulent SVCV<\/jats:styled-content> challenge resulting in a high relative per cent survival of 82.5%.<\/jats:p>","DOI":"10.1111\/jfd.12678","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T11:22:49Z","timestamp":1502450569000},"page":"67-78","source":"Crossref","is-referenced-by-count":13,"title":["Virulence of a chimeric recombinant infectious haematopoietic necrosis virus expressing the spring viraemia of carp virus glycoprotein in salmonid and cyprinid fish"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5217-6030","authenticated-orcid":false,"given":"E J","family":"Emmenegger","sequence":"first","affiliation":[{"name":"US Geological Survey Western Fisheries Research Center Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Biacchesi","sequence":"additional","affiliation":[{"name":"Virologie et Immunologie Mol\u00e9culaires (VIM) INRA Universit\u00e9 Paris\u2010Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"M\u00e9rour","sequence":"additional","affiliation":[{"name":"Virologie et Immunologie Mol\u00e9culaires (VIM) INRA Universit\u00e9 Paris\u2010Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Glenn","sequence":"additional","affiliation":[{"name":"NanoString Technologies Inc. Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A D","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chemical and Life Sciences Laboratories University of Illinois at Urbana\u2010Champaign Urbana IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Br\u00e9mont","sequence":"additional","affiliation":[{"name":"Virologie et Immunologie Mol\u00e9culaires (VIM) INRA Universit\u00e9 Paris\u2010Saclay Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Kurath","sequence":"additional","affiliation":[{"name":"US Geological Survey Western Fisheries Research Center Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12678","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T21:41:44Z","timestamp":1696023704000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1111\/jfd.12678"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12678","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}