{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:12:56Z","timestamp":1789747976319,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011262","name":"FP7 Food, Agriculture and Fisheries, Biotechnology","doi-asserted-by":"publisher","award":["613611"],"award-info":[{"award-number":["613611"]}],"id":[{"id":"10.13039\/100011262","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The availability of a rapid and accurate method for the diagnosis of\n                    <jats:italic>Photobacterium damselae<\/jats:italic>\n                    subsp.\n                    <jats:italic>piscicida<\/jats:italic>\n                    (\n                    <jats:italic>Phdp<\/jats:italic>\n                    ), able to discriminate its strictly correlated subsp.\n                    <jats:italic>damselae<\/jats:italic>\n                    (\n                    <jats:italic>Phdd<\/jats:italic>\n                    ), formally known as\n                    <jats:italic>Vibrio damsela<\/jats:italic>\n                    , is essential for managing fish pasteurellosis outbreaks in farmed fish. A single\u2010step, high\u2010sensitivity real\u2010time\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    assay for simultaneous detection and quantification of\n                    <jats:italic>P.\u00a0damselae<\/jats:italic>\n                    was designed targeting partial of the sequence of the\n                    <jats:italic>bamB<\/jats:italic>\n                    gene and tested for specificity and sensitivity on laboratory\u2010generated samples as well as on experimentally infected seabream tissue samples. With a limit of detection (\n                    <jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content>\n                    ) of one copy in pure bacterial\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    , the sensitivity was higher than all methods previously reported. Validation in target and non\u2010target bacterial species proved the assay was able to discriminate\n                    <jats:italic>Phdd<\/jats:italic>\n                    \u2010\n                    <jats:italic>Phdp<\/jats:italic>\n                    subspecies from diverse hosts\/geographical origins and between non\u2010target species. In addition, two\n                    <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>\n                    s in the target amplicon region determine two distinctive\n                    <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>\n                    dissociation curves distinguishing between\n                    <jats:italic>Phdp<\/jats:italic>\n                    \u2010\n                    <jats:italic>Phdd<\/jats:italic>\n                    . This is the first time that a molecular method for\n                    <jats:italic>P.\u00a0damselae<\/jats:italic>\n                    diagnosis combines detection, quantification and subspecies identification in one step. The assay holds the potential to improve the knowledge of infection dynamics and the development of better strategies to control an important fish disease.\n                  <\/jats:p>","DOI":"10.1111\/jfd.12703","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T07:41:53Z","timestamp":1504165313000},"page":"247-254","source":"Crossref","is-referenced-by-count":18,"title":["Development of a real\u2010time\n                    <scp>PCR<\/scp>\n                    assay for rapid detection and quantification of\n                    <i>Photobacterium damselae<\/i>\n                    subsp.\n                    <i>piscicida<\/i>\n                    in fish tissues"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5476-6154","authenticated-orcid":false,"given":"R","family":"Carraro","sequence":"first","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Dalla Rovere","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ferraresso","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Carraro","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Franch","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Toffan","sequence":"additional","affiliation":[{"name":"Fish Virology Department National Reference Laboratory for Fish Crustacean and Mollusc Diseases Istituto Zooprofilattico Sperimentale delle Venezie Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Pascoli","sequence":"additional","affiliation":[{"name":"Fish Virology Department National Reference Laboratory for Fish Crustacean and Mollusc Diseases Istituto Zooprofilattico Sperimentale delle Venezie Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Patarnello","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Bargelloni","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijvsm.2013.10.004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12237"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2009.01027.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/793817"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.10.022"},{"key":"e_1_2_6_7_1","first-page":"72","article-title":"Comparative histopathological and immunohistochemical evaluations in juvenile sea bass (Dicentrarhus labrax) and gilthead sea bream (Sparus aurata) naturally infected with Photobacterium damselae subsp. piscicida","volume":"164","author":"Avci H.","year":"2013","journal-title":"Revue de Medecine Veterinaire"},{"key":"e_1_2_6_8_1","first-page":"75","article-title":"Update on Bacterial Vaccines: Photobacterium damselae subsp. piscicida","volume":"121","author":"Barnes A. 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