{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:11:07Z","timestamp":1791515467900,"version":"4.3.4"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T00:00:00Z","timestamp":1527465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2017M611534"],"award-info":[{"award-number":["2017M611534"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS\u201045\u201019"],"award-info":[{"award-number":["CARS\u201045\u201019"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Herpesviral haematopoietic necrosis (<jats:styled-content style=\"fixed-case\">HVHN<\/jats:styled-content>), caused by cyprinid herpesvirus 2 (Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102), causes significant losses in crucian carp (<jats:italic>Carassius carassius<\/jats:italic>) aquaculture. Rapid and convenient <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> assay detection of Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102 is useful for field diagnosis. Recombinase polymerase amplification (<jats:styled-content style=\"fixed-case\">RPA<\/jats:styled-content>) is a novel isothermal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> amplification and detection technology that can amplify <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> within 30\u00a0min at ~37\u00b0C by simulating in\u00a0vivo <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> recombination. Herein, a rapid and convenient detection assay based on <jats:styled-content style=\"fixed-case\">RPA<\/jats:styled-content> with a lateral flow dipstick (<jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content>) was developed for detecting Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102. The highly conserved <jats:italic><jats:styled-content style=\"fixed-case\">ORF<\/jats:styled-content>72<\/jats:italic> of Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102 was targeted by specific and sensitive primers and probes. The optimized assay takes only 15\u00a0min at 38\u00b0C using a water bath, with analysis of products by 2% agarose gel electrophoresis within 30\u00a0min. A simple lateral flow strip based on the unique probe in reaction buffer was developed for visualization. The entire <jats:styled-content style=\"fixed-case\">RPA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> assay takes 50\u00a0min less than the routine <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> method, is 100 times more sensitive and displays no cross\u2010reaction with other aquatic viruses. The combined isothermal <jats:styled-content style=\"fixed-case\">RPA<\/jats:styled-content> and lateral flow assay (<jats:styled-content style=\"fixed-case\">RPA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content>) provides a simple, rapid, reliable method that could improve field diagnosis of Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102 when resources are limited.<\/jats:p>","DOI":"10.1111\/jfd.12808","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T07:09:31Z","timestamp":1527491371000},"page":"1201-1206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8893-1568","authenticated-orcid":false,"given":"H","family":"Wang","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University Shanghai China"},{"name":"Shanghai Engineering Research Center of Aquatic Product Processing &amp; Preservation Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Sun","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Xu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Podok","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Technology Phuket Rajabhat University Phuket Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Xie","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Aquatic Product Processing &amp; Preservation Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ys","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5665-7157","authenticated-orcid":false,"given":"Lq","family":"Lu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University Shanghai China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture Shanghai Ocean University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2014.11.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03206-12"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1577\/H05-007.1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao069137"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.00988.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2014.08.006"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1995.tb00296.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12648"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2014-0796"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17269-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.12.006"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-9-2399"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2004.tb12650.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12340"},{"key":"e_1_2_6_16_1","first-page":"164","article-title":"Mass mortality caused by cyprinid herpesvirus 2 (CyHV\u20102) in prussian carp (Carassius gibelio) in China","volume":"32","author":"Wang L.","year":"2012","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.07.004"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2011-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.05.025"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T03:51:14Z","timestamp":1695009074000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,28]]},"references-count":18,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1111\/jfd.12808"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12808","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,28]]},"assertion":[{"value":"2018-02-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}