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In 2015, <jats:italic>infectious spleen and kidney necrosis virus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content>) was detected in zebrafish from a research facility in Spain. Affected fish showed lethargy, loss of appetite, abnormal swimming, distention of the coelomic cavity and, in the most severe cases, respiratory distress, pale gills and petechial haemorrhages at the base of fins. Cytomegaly was the most relevant histopathological finding in organs and tissues, sometimes associated to degenerative and necrotic changes. <jats:styled-content style=\"fixed-case\">ISKNV<\/jats:styled-content> belongs to the relatively newly defined genus <jats:italic>Megalocytivirus<\/jats:italic>, family <jats:italic>Iridoviridae,<\/jats:italic> comprising large, icosahedral cytoplasmic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> viruses. This is the first case of naturally occurring <jats:italic>Megalocytivirus<\/jats:italic> infection in zebrafish research facilities, associated with morbidity. The virus has been identified based on both pathologic and genetic evidence, to better understand the pathogenesis of the infection in zebrafish and the phylogenetic relationship with other iridoviruses. Given the ability of megalocytiviruses to cross\u2010species boundaries, it seems necessary to implement stringent biosecurity practices as these infections may invalidate experimental data and have major impact on laboratory and cultured fish.<\/jats:p>","DOI":"10.1111\/jfd.12822","type":"journal-article","created":{"date-parts":[[2018,6,8]],"date-time":"2018-06-08T06:04:04Z","timestamp":1528437844000},"page":"1283-1294","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["First description of a natural infection with spleen and kidney necrosis virus in zebrafish"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4969-4122","authenticated-orcid":false,"given":"Roberto","family":"Berm\u00fadez","sequence":"first","affiliation":[{"name":"Department of Anatomy, Animal Production and Veterinary Clinical Sciences Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-0352","authenticated-orcid":false,"given":"Ana Paula","family":"Losada","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Production and Veterinary Clinical Sciences Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2451-5039","authenticated-orcid":false,"given":"Ana Manuela","family":"de Azevedo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Production and Veterinary Clinical Sciences Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8365-7125","authenticated-orcid":false,"given":"Jorge","family":"Guerra\u2010Varela","sequence":"additional","affiliation":[{"name":"Department of Zoology, Genetics and Physical Anthropology Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"P\u00e9rez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Zoology, Genetics and Physical Anthropology Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Zoology, Genetics and Physical Anthropology Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8610-5692","authenticated-orcid":false,"given":"Francesc","family":"Padr\u00f3s","sequence":"additional","affiliation":[{"name":"Department of Animal Biology, Vegetal Biology and Ecology Autonomous University of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Nowak","sequence":"additional","affiliation":[{"name":"Institute for Marine and Antarctic Studies University of Tasmania Hobart Tasmania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Isabel","family":"Quiroga","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Production and Veterinary Clinical Sciences Faculty of Veterinary University of Santiago de Compostela Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901495"},{"key":"e_1_2_6_3_1","first-page":"363","article-title":"High mortality due to Tetrahymena sp. infection in laboratory\u2010maintained zebrafish (Brachydanio rerio)","volume":"52","author":"Astrofsky K. 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