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Currently there is no vaccine available for protection against <jats:styled-content style=\"fixed-case\">HSMI<\/jats:styled-content>, partly due to the lack of a cell line for efficient virus propagation. Erythrocytes are the primary target cells for <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content> in\u00a0vivo and a potential source for isolation of <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content> particles. In this study, <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content> was purified from infected erythrocytes, inactivated and used in a vaccination trial against <jats:styled-content style=\"fixed-case\">HSMI<\/jats:styled-content>. A single immunization with adjuvanted, inactivated <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content> induced protection against <jats:styled-content style=\"fixed-case\">HSMI<\/jats:styled-content> in Atlantic salmon infected by virus injection 6\u00a0weeks later, while a moderate protection was obtained in fish infected through natural transmission, i.e. cohabitation. The <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content> vaccine significantly reduced <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content> loads and histopathological lesions typical for <jats:styled-content style=\"fixed-case\">HSMI<\/jats:styled-content> compared to the unvaccinated control group. This is the first demonstration of protective vaccination against <jats:styled-content style=\"fixed-case\">PRV<\/jats:styled-content>, and promising for future control of <jats:styled-content style=\"fixed-case\">HSMI<\/jats:styled-content> in Atlantic salmon aquaculture.<\/jats:p>","DOI":"10.1111\/jfd.12835","type":"journal-article","created":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T11:03:56Z","timestamp":1529579036000},"page":"1411-1419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Inactivated <i>Piscine orthoreovirus<\/i> vaccine protects against heart and skeletal muscle inflammation in Atlantic salmon"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0811-7305","authenticated-orcid":false,"given":"\u00d8ystein","family":"Wessel","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Norwegian University of Life Sciences Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8yvind","family":"Haugland","sequence":"additional","affiliation":[{"name":"PHARMAQ Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marit","family":"Rode","sequence":"additional","affiliation":[{"name":"PHARMAQ Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00f8rge N.","family":"Fredriksen","sequence":"additional","affiliation":[{"name":"PHARMAQ Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria K.","family":"Dahle","sequence":"additional","affiliation":[{"name":"Department of Fish Health Norwegian Veterinary Institute Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9911-5948","authenticated-orcid":false,"given":"Espen","family":"Rimstad","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Norwegian University of Life Sciences Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc15c01s14"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0244-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171471"},{"key":"e_1_2_7_5_1","volume-title":"The state of world fisheries and aquaculture 2016","author":"FAO","year":"2016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2004.00602.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-45-35"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-27"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146229"},{"issue":"98","key":"e_1_2_7_10_1","article-title":"First description of clinical presentation of piscine orthoreovirus (PRV) infections in salmonid aquaculture in Chile and identification of a second genotype (Genotype II) of PRV","volume":"13","author":"Godoy M. 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