{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T15:45:29Z","timestamp":1789227929957,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T00:00:00Z","timestamp":1534118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Information about motile aeromonads from aquaculture systems of the Neotropical region is scarce. The aim of this study was to characterize motile <jats:italic>Aeromonas<\/jats:italic> isolated from ornamental and consumable fishes cultured in Uruguay. Biochemical and molecular methods were used for species identification. Antimicrobial susceptibility and the presence of virulence genes were evaluated. Genetic diversity was analysed by rep\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, and virulence of the most representative isolates was determined by calculating the fifty lethal dose in experimentally challenged fish (<jats:italic>Australoheros facetus<\/jats:italic>). <jats:italic>Aeromonas hydrophila<\/jats:italic> and <jats:italic>A.\u00a0veronii<\/jats:italic> were the most prevalent identified species (38.2% and 32.4%, respectively), whereas <jats:italic>A.\u00a0allosacharophila<\/jats:italic>,<jats:italic> A.\u00a0bestiarium<\/jats:italic>,<jats:italic> A.\u00a0caviae<\/jats:italic> and <jats:italic>A.\u00a0punctata<\/jats:italic> were less prevalent. This study constitutes the first report of these last four species in Uruguay. All isolates were resistant to at least three antimicrobials, and 82.3% of them showed multidrug resistance. Virulence genotypes were correlated with the <jats:italic>Aeromonas<\/jats:italic> species and haemolytic activity. The genotype <jats:italic>act<\/jats:italic>+\/<jats:italic>alt<\/jats:italic>+\/<jats:italic>ast<\/jats:italic>+\/<jats:italic>ela<\/jats:italic>+\/<jats:italic>lip<\/jats:italic>+ was the most prevalent (26.5%). A correlation between virulence genotypes and <jats:italic>Aeromonas<\/jats:italic> species was found. <jats:italic>A.\u00a0punctata<\/jats:italic> showed a clonal structure according to rep\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis, whereas other species showed high genetic diversity. The number of virulence genes of the isolates was related with virulence according to the experimental challenge assays.<\/jats:p>","DOI":"10.1111\/jfd.12864","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T06:10:33Z","timestamp":1534227033000},"page":"1559-1569","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Phenotypic, molecular and pathological characterization of motile aeromonads isolated from diseased fishes cultured in Uruguay"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2318-3906","authenticated-orcid":false,"given":"Alejandro","family":"Perretta","sequence":"first","affiliation":[{"name":"Facultad de Veterinaria Instituto de Investigaciones Pesqueras Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Ant\u00fanez","sequence":"additional","affiliation":[{"name":"Department of Microbiology Instituto de Investigaciones Biol\u00f3gicas \u201cClemente Estable\u201d Ministerio de Educaci\u00f3n y Cultura Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Zunino","sequence":"additional","affiliation":[{"name":"Department of Microbiology Instituto de Investigaciones Biol\u00f3gicas \u201cClemente Estable\u201d Ministerio de Educaci\u00f3n y Cultura Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2348-2357.2003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4172\/2325-9841.1000115"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.11.011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-1089-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.04.012"},{"issue":"1","key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"e0115813","DOI":"10.1371\/journal.pone.0115813","article-title":"Strategies to avoid wrongly labelled genomes using as example the detected wrong taxonomic affiliation for Aeromonas genomes in the GenBank database","volume":"10","author":"Baez\u2010Hidalgo R.","year":"2015","journal-title":"PlosONE"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/m77-174"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12628"},{"issue":"177","key":"e_1_2_6_12_1","first-page":"27","article-title":"Caracterizaci\u00f3n de septicemia hemorr\u00e1gica bacteriana (SHB), diagnosticada en peces ornamentales de Uruguay","volume":"46","author":"Carnevia D.","year":"2010","journal-title":"Veterinaria (Montevideo)"},{"key":"e_1_2_6_13_1","article-title":"Relevamiento de pisciculturas ornamentales en Uruguay","author":"Carnevia D.","year":"2013","journal-title":"Actas del Primer Congreso Nacional de Veterinaria"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-009-9335-6"},{"key":"e_1_2_6_15_1","first-page":"68","volume-title":"Aeromonas hydrophila and motile aeromonad septicemias of fish","author":"Cipriano R.","year":"2001"},{"key":"e_1_2_6_16_1","unstructured":"CLSI. (2006).Clinical and Laboratory Standard Institute. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated and Fastidious Bacteria; Proposed Guideline. Document: M45\u2010P. Pennsylvania."},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1244"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2012.12.005"},{"key":"e_1_2_6_19_1","unstructured":"DINARA. (2014).Bolet\u00edn Estad\u00edstico Pesquero zafra 2014.http:\/\/www.mgap.gub.uy\/sites\/default\/files\/multimedia\/boletin_estadistico_pesquero_2014.pdf(accessed 18 nov 2017)"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2008.12.015"},{"key":"e_1_2_6_21_1","unstructured":"FAO. (2016).El estado mundial de la pesca y la acuicultura 2016. Contribuci\u00f3n a la seguridad alimentaria y la nutrici\u00f3n para todos. Roma."},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_6_23_1","volume-title":"Probit Analysis, a statistical treatment of the sigmoid response curve","author":"Finney D.","year":"1947"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13559.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00848-14"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.3574"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02733"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00039-09"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8030(94)90033-7"},{"issue":"2","key":"e_1_2_6_31_1","first-page":"76","article-title":"Multidrug resistance in Aeromonas hydrophila isolates of fish","volume":"3","author":"Kaskhedikar M.","year":"2010","journal-title":"Veterinary World"},{"issue":"12","key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"5293","DOI":"10.1128\/AEM.65.12.5293-5302.1999","article-title":"PCR detection, characterization, and distribution of virulence genes in Aeromonas spp","volume":"65","author":"Kingdome C.","year":"1999","journal-title":"Applied Environmental Microbiology"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01784.x"},{"issue":"1","key":"e_1_2_6_34_1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/aem.46.1.165-170.1983","article-title":"Multiple antibiotic resistance indexing of Escherichia coli to identify high\u2010risk sources of fecal contamination of foods","volume":"46","author":"Krumperman P.","year":"1983","journal-title":"Applied Environmental Microbiology"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9320-4"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2011.04944.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-28022-7_12"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-3-412"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2010.11.014"},{"key":"e_1_2_6_40_1","unstructured":"Metz J.(2015).Delineation of Aeromonas hydrophila pathotypes by dectection of putative virulence factors using polymerase chain reaction and nematode challenge assay. MSc. dissertation Kennesaw State University Georgia."},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13887"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0982-3"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765X.2003.01410.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01337"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01615"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.12.5675-5682.2001"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02398.x"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1006-8104(13)60005-7"},{"key":"e_1_2_6_49_1","volume-title":"Numerical taxonomy","author":"Sneath P.","year":"1973"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.03048-0"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_2_6_53_1","first-page":"25","article-title":"Genomic fingerprinting of bacteria using repetitive sequence\u2010based polymerase chain reaction","volume":"5","author":"Versalovic J.","year":"1994","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02443-0"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aqrep.2015.11.003"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12864","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12864","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T12:14:29Z","timestamp":1695039269000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12864"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,13]]},"references-count":54,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/jfd.12864"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12864","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,13]]},"assertion":[{"value":"2018-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}