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The use of double\u2010strength <jats:italic>Salmonella\u2010Shigella<\/jats:italic> (<jats:styled-content style=\"fixed-case\">DSSS<\/jats:styled-content>) broth and <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> agar yielded <jats:italic>Edwardsiella<\/jats:italic> isolation from intestine in 100% of those edwardsiellosis\u2010diseased eels, but also in 40.4% of other sick fish with vibriosis or aeromonosis and in 28.8% of healthy eels, as well as from freshwater in 8.3% of samples. Pure cultures were isolated on <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> agar from the former, but motile <jats:italic>Aeromonas<\/jats:italic>,<jats:italic> Plesiomonas shigelloides<\/jats:italic> and <jats:italic>Hafnia alvei<\/jats:italic> were recovered along with <jats:italic>Edwardsiella<\/jats:italic> in the other samples. <jats:italic>Edwardsiella<\/jats:italic> isolates identification at species level revealed that <jats:italic>E.\u00a0piscicida<\/jats:italic> was distributed between wild eels and freshwater but <jats:italic>E.\u00a0tarda<\/jats:italic> only did in freshwater. All <jats:italic>E.\u00a0piscicida<\/jats:italic> strains were virulent for eels (<jats:styled-content style=\"fixed-case\">LD<\/jats:styled-content><jats:sub>\u00a0<\/jats:sub><jats:styled-content>&lt;<\/jats:styled-content>\u00a01.0\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/fish) but that of <jats:italic>E.\u00a0tarda<\/jats:italic> was not. This is the first report of <jats:italic>E.\u00a0piscicida<\/jats:italic> in wild eel intestines and natural freshwater, highlighting its role as potential reservoirs for the bacterium. A seasonal recovery was found for <jats:italic>E.\u00a0piscicida<\/jats:italic> at water temperature above 20\u00b0C.<\/jats:p>","DOI":"10.1111\/jfd.12867","type":"journal-article","created":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T09:29:25Z","timestamp":1532424565000},"page":"1613-1623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Seasonal recovery of <i>Edwardsiella piscicida<\/i> from wild European eels and natural waters: Isolation methods, virulence and reservoirs"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7053-0408","authenticated-orcid":false,"given":"Consuelo","family":"Esteve","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Ecolog\u00eda Universitat de Val\u00e8ncia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Alcaide","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Ecolog\u00eda Universitat de Val\u00e8ncia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.03.006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12080"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao073077"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.43.6.1380-1384.1982"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(1993)005<0189:POESOC>2.3.CO;2"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-33.3.517"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1991.tb00581.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2017.09.004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2006.00750.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02203"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.179150"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80432-7"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2008.12.015"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2210-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00519.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2011.10.002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao016015"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12574"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-15-1-33"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/005405-0"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-29.2.334"},{"key":"e_1_2_8_23_1","volume-title":"Standard methods for the examination of water and wastewater","author":"Greenberg A.E.","year":"1992"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.03.027"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00241-4"},{"key":"e_1_2_8_26_1","first-page":"178","volume-title":"Bergey\u2032s Manual of Determinative Bacteriology 9th edition","author":"Holt J. 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