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Antimicrobial treatment remains the primary means of control; however, there are limited choices available for use. The objectives of the study were therefore to determine the minimum inhibitory concentrations for erythromycin and florfenicol in selected <jats:italic>F.\u00a0psychrophilum<\/jats:italic> isolates and to evaluate their clinical treatment efficacy in experimentally infected rainbow trout. All isolates tested had moderate susceptibility to florfenicol and erythromycin except one isolate, which had low susceptibility to erythromycin. Two isolates (one with moderate and one with low susceptibility to erythromycin) were used in an experimental infection trial. Rainbow trout juveniles were injected intraperitoneally with 10<jats:sup>8<\/jats:sup> cfu\/fish and after mortality had begun, fish were given erythromycin\u2010 and florfenicol\u2010medicated feed at a rate of 75\u00a0mg kg<jats:sup>\u2212<\/jats:sup><jats:sup>1<\/jats:sup> day<jats:sup>\u2212<\/jats:sup><jats:sup>1<\/jats:sup> and 10\u00a0mg kg<jats:sup>\u2212<\/jats:sup><jats:sup>1<\/jats:sup>\u00a0day<jats:sup>\u2212<\/jats:sup><jats:sup>1<\/jats:sup> fish body weight, respectively, for 10 consecutive days. The splenic <jats:italic>F.\u00a0psychrophilum<\/jats:italic> load was determined using an <jats:italic>rpoC<\/jats:italic> quantitative PCR throughout the 30\u2010day trial. Relative to antibiotic\u2010free controls, erythromycin treatment significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) reduced mortality of rainbow trout juveniles infected with FPG101, even when treatment was initiated after clinical signs developed.<\/jats:p>","DOI":"10.1111\/jfd.12944","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T10:17:25Z","timestamp":1547201845000},"page":"325-334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Erythromycin and florfenicol treatment of rainbow trout <i>Oncorhynchus mykiss<\/i> (Walbaum) experimentally infected with <i>Flavobacterium psychrophilum<\/i>"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7896-8498","authenticated-orcid":false,"given":"Maureen","family":"Jarau","sequence":"first","affiliation":[{"name":"Fish Pathology Laboratory University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet I.","family":"MacInnes","sequence":"additional","affiliation":[{"name":"Department of Pathobiology University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3455-4197","authenticated-orcid":false,"given":"John S.","family":"Lumsden","sequence":"additional","affiliation":[{"name":"Fish Pathology Laboratory University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.78.1.25-32.1959"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1577\/H08-014.1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(01)00897-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(00)00310-0"},{"key":"e_1_2_6_6_1","volume-title":"Methods for broth dilution susceptibility testing for bacteria isolated from aquatic animals: Approved guideline. 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