{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:58:18Z","timestamp":1791853098480,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T00:00:00Z","timestamp":1547510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several isolates of an unknown oomycete resembling the genus <jats:italic>Aphanomyces<\/jats:italic> were obtained into laboratory culture from samples of noble crayfish (<jats:italic>Astacus astacus<\/jats:italic>) in 2016\u20132017. The crayfish were kept in cages in connection with a study on an eventually persistent crayfish plague infection in a small Finnish lake, following an acute episode of the disease in 2010. Despite the close resemblance of the isolates to the causative agent of crayfish plague, <jats:italic>Aphanomyces astaci<\/jats:italic>, and the positive results obtained in OIE recommended <jats:italic>A.\u00a0astaci<\/jats:italic>\u2010specific ITS\u2010based conventional PCR and qPCR molecular assays, the isolates can be distinguished from <jats:italic>A.\u00a0astaci<\/jats:italic> by morphological features concerning hyphal structure and chlamydospore formation, as well as using the randomly amplified polymorphic DNA\u2010polymerase chain reaction (RAPD\u2010PCR) method, microsatellite\u2010based genotyping, the pathogenicity test and phylogenetic analysis based on ITS sequencing. The name <jats:italic>Aphanomyces fennicus <\/jats:italic>sp. novum is proposed for this close relative of <jats:italic>A.\u00a0astaci<\/jats:italic>. The detection of this tentative novel species giving false\u2010positive results in existing diagnostic assays for the crayfish plague highlights the importance of careful interpretation of the results from molecular methods, especially concerning crayfish with low\u2010level infections, excluding the possibility to verify the results from clinical or sequencing data.<\/jats:p>","DOI":"10.1111\/jfd.12955","type":"journal-article","created":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T09:47:53Z","timestamp":1547545673000},"page":"413-422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["A tentative new species <i>Aphanomyces fennicus<\/i> sp. nov. interferes with molecular diagnostic methods for crayfish plague"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1172-8852","authenticated-orcid":false,"given":"Satu","family":"Viljamaa\u2010Dirks","sequence":"first","affiliation":[{"name":"Veterinary Bacteriology and Pathology Research Unit, OIE Reference Laboratory for Crayfish Plague Finnish Food Safety Authority Evira Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirpa","family":"Heinikainen","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology and Pathology Research Unit, OIE Reference Laboratory for Crayfish Plague Finnish Food Safety Authority Evira Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1986.tb01030.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1051\/kmae:2007002"},{"key":"e_1_2_7_4_1","first-page":"335","article-title":"First isolation of Aphanomyces frigidophilus (Saprolegniales) in Europe","volume":"95","author":"Ballesteros I.","year":"2006","journal-title":"Mycotaxon"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03275"},{"key":"e_1_2_7_6_1","first-page":"131","article-title":"The crayfish plague fungus, Aphanomyces astaci \u2013 Diagnosis, isolation and pathobiology","volume":"7","author":"Cerenius L.","year":"1988","journal-title":"Freshwater Crayfish"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0953-7562(09)80716-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.02.004"},{"key":"e_1_2_7_9_1","article-title":"Introduction to oomycetes","author":"Fry W. 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