{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T23:28:07Z","timestamp":1767914887736,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,10]],"date-time":"2019-03-10T00:00:00Z","timestamp":1552176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A030313179"],"award-info":[{"award-number":["2017A030313179"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Nocardia<\/jats:italic><jats:italic> seriolae<\/jats:italic>, a facultative intracellular bacterium, is the main pathogen of fish nocardiosis. Bioinformatic analysis showed that the histone\u2010like DNA\u2010binding protein (HLP) gene of <jats:italic>N.\u00a0seriolae<\/jats:italic> (<jats:italic>nshlp<\/jats:italic>) encoded a secreted protein and might target the mitochondria in the host cell. To further study the preliminary function of HLP in <jats:italic>N.\u00a0seriolae <\/jats:italic>(NsHLP), the gene cloning, extracellular products identification, subcellular localization, overexpression and apoptosis detection assay were carried out in this study. Mass spectrometry analysis of the extracellular products from <jats:italic>N.\u00a0seriolae<\/jats:italic> showed that NsHLP was a secreted protein. Subcellular localization of HLP\u2010GFP fusion proteins mainly assembled in the nucleus, which indicated that the NsHLP was co\u2010located with the nucleus rather than mitochondria in fathead minnow (FHM) cells. Notably, the expression of NsHLP had changed the distribution of mitochondria into lumps in the FHM cell. In addition, apoptotic features were found in the transfected FHM cells by overexpression of NsHLP. Quantitative assays of mitochondrial membrane potential value, caspase\u20103 activity and pro\u2010apoptotic genes mRNA (<jats:italic>Bad<\/jats:italic>, <jats:italic>Bid<\/jats:italic> and <jats:italic>Bax<\/jats:italic>) expression level demonstrated that the cell apoptosis was induced in the transfected FHM cells. All the results presented in this study provided insight on the function of NsHLP, which suggested that it may participate in the cell apoptosis regulation and play an important role in the pathogenesis of <jats:italic>N<\/jats:italic>.\u00a0<jats:italic>seriolae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.12962","type":"journal-article","created":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T06:05:06Z","timestamp":1552284306000},"page":"657-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification and functional characterization of Histone\u2010like DNA\u2010binding protein in <i>Nocardia seriolae<\/i> (NsHLP) involved in cell apoptosis"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1662-2130","authenticated-orcid":false,"given":"Wenji","family":"Wang","sequence":"first","affiliation":[{"name":"Shenzhen Research Institute of Guangdong Ocean University 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