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Their co\u2010infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross\u2010protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV\u2010N438A\u2010\u0394NV\u2010EGFP and rIHNV\u2010N438A\u2010\u0394NV\u2010VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (<jats:italic>EGFP<\/jats:italic>) reporter gene and antigenic <jats:italic>VP2<\/jats:italic> gene of IPNV. Meanwhile, we tested their efficacy against the wild\u2010type (wt) IHNV HLJ\u201009 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ\u201009 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV\u2010N438A\u2010\u0394NV\u2010VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine.<\/jats:p>","DOI":"10.1111\/jfd.12966","type":"journal-article","created":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T11:04:43Z","timestamp":1552647883000},"page":"631-642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)\u2010G<sup>N438A<\/sup> expresses <i>VP2<\/i> gene\u2010encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9732-7277","authenticated-orcid":false,"given":"Yaping","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology 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