{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T19:05:01Z","timestamp":1773774301760,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T00:00:00Z","timestamp":1549843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study reports on the characterization of <jats:italic>Vagococcus salmoninarum<\/jats:italic> using phenotypic, serological, antigenic, genetic and proteomic methods. All strains of <jats:italic>V.\u00a0salmoninarum<\/jats:italic> were resistant to most of the antimicrobials tested, and only 10% of strains were sensitive to florfenicol. Serological analysis demonstrated a high antigenic homogeneity within the species. No cross\u2010reaction was detected with other fish pathogenic species causing streptococcosis (<jats:italic>Lactococcus garvieae<\/jats:italic>, <jats:italic>Streptococcus parauberis<\/jats:italic>, <jats:italic>Streptococcus ini<\/jats:italic>ae, <jats:italic>Streptococcus agalactiae<\/jats:italic>, <jats:italic>Carnobacterium maltaromaticum<\/jats:italic>) using serum against <jats:italic>V.\u00a0salmoninarum<\/jats:italic> CECT 5810. Electrophoretic analysis of cell surface proteins and immunoblot supported the antigenic homogeneity within <jats:italic>V.\u00a0salmoninarum<\/jats:italic> strains. Moreover, limited diversity was detected using genomic (RAPD, ERIC\u2010PCR and REP\u2010PCR) and MALDI\u2010TOF\u2010MS analyses. The phenotypic, genomic and proteomic methods tested allowed the rapid differentiation of <jats:italic>V.\u00a0salmoninarum<\/jats:italic> from the other species causing streptococcosis. However, MALDI\u2010TOF\u2010MS is the most promising method for typing and characterization of <jats:italic>V.\u00a0salmoninarum<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.12967","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T17:11:53Z","timestamp":1549905113000},"page":"597-612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification and typing of <i>Vagococcus salmoninarum <\/i>using genomic and proteomic techniques"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7959-1645","authenticated-orcid":false,"given":"Yolanda","family":"Torres\u2010Corral","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Edificio CIBUS Facultad de Biolog\u00eda and Instituto de Investigaci\u00f3n y An\u00e1lisis Alimentario Universidad de Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4398-4102","authenticated-orcid":false,"given":"Ysabel","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Edificio CIBUS Facultad de Biolog\u00eda and Instituto de Investigaci\u00f3n y An\u00e1lisis Alimentario Universidad de Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.03.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.06.032"},{"issue":"3","key":"e_1_2_7_4_1","first-page":"375","article-title":"Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of Lactococcus garvieae strains","volume":"19","author":"Altun S.","year":"2013","journal-title":"Kafkas Universitesi Veteriner Fakultesi Dergisi"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12210"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01492"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-32674-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02296"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201200532"},{"key":"e_1_2_7_10_1","volume-title":"Bacteria and Fungi from fish and other aquatic animals","author":"Buller N.","year":"2014"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-016-0794-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-41"},{"issue":"6","key":"e_1_2_7_13_1","first-page":"235","article-title":"First isolation of Vagococcus salmoninarum from cultured rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in Turkey","volume":"31","author":"Didinen B. 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