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Aqueous fixatives demonstrated excellent cytological preservation but failed to deliver the preservation of the mucus when compared to the non\u2010aqueous\u2010based fixatives; qualitative and semi\u2010quantitative analysis revealed a greater preservation of the gill mucus using the non\u2010aqueous methacarn solution. A combination of this fixation method and an Alcian blue\/Periodic acid\u2013Schiff staining was tested in gills of Atlantic salmon infected with amoebic gill disease; lectin labelling was also used to confirm the mucus preservation in the methacarn\u2010fixed tissue. Amoebae were observed closely associated with the mucus demonstrating that the techniques employed for preservation of the mucous coat can indeed avoid the loss of potential mucus\u2010embedded parasites, thus providing a better understanding of the relationship between the mucus and parasite.<\/jats:p>","DOI":"10.1111\/jfd.12988","type":"journal-article","created":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T00:54:50Z","timestamp":1554512090000},"page":"883-894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Methacarn preserves mucus integrity and improves visualization of amoebae in gills of Atlantic salmon (<i>Salmo salar<\/i> L.)"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5896-8186","authenticated-orcid":false,"given":"Carolina","family":"Fernandez","sequence":"first","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dario","family":"Mascolo","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean J.","family":"Monaghan","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna L.","family":"Baily","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn","family":"Chalmers","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Paladini","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Bron","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Fridman","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture, Faculty of Natural Sciences University of Stirling Stirling Scotland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,4]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1046\/j.1365-2761.2001.00330.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1046\/j.1365-2761.2003.00496.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.1002\/(SICI)1097-0029(19970301)36:5<390::AID-JEMT9>3.0.CO;2-P"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1016\/j.vetmic.2014.03.008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1111\/j.1439-0426.1991.tb00523.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.1007\/BF01299812"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_1","DOI":"10.1128\/aem.62.10.3650-3654.1996"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_1","DOI":"10.1039\/C5MB00890E"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1111\/j.1095-8649.1984.tb04845.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.1111\/jfd.12078"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.1017\/S0031182017000622"},{"key":"e_1_2_7_13_1","first-page":"187","article-title":"Characterization of mucus glycoproteins in the intestinal mucosa of the African elephant (Loxodonta africana) following lectin histochemistry","volume":"62","author":"Coet\u2010Zee H. 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