{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T04:40:17Z","timestamp":1772080817931,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T00:00:00Z","timestamp":1554681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15H04544"],"award-info":[{"award-number":["15H04544"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cyprinid herpesvirus 2 (Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102) is the causative agent of herpesviral haematopoietic necrosis (<jats:styled-content style=\"fixed-case\">HVHN<\/jats:styled-content>) in goldfish, <jats:italic>Carassius auratus<\/jats:italic>, and Prussian carp, <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>auratus gibelio<\/jats:italic>. In this study, we investigated virus persistence in goldfish experimentally infected with Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102. Virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> presence in organs was monitored in survivors reared at a virus permissive temperature and also in survivors treated with a non\u2010permissive temperature for 4\u00a0days, initiated at three different time points post\u2010infection in order to obtain fish with different virus loads. We detected virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> in all organs tested at 51\u00a0days post\u2010infection (dpi) and in the spleen, trunk kidney and gills of survivors at 81 dpi, although the virus load in fish influenced the subsequent number of organs that tested positive for virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. In addition, some organs dissected from four out of five asymptomatic survivors tested positive by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> following incubation in vitro in a medium for 5\u00a0days. Following inoculation with the homogenate of <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010positive kidney incubated in vitro, one of the three inoculated fish died, showing that the detected virus by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> produced infectious particles. This study suggests that Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>\u20102 can establish a persistent infection in some organs, especially the spleen and trunk kidney, and that asymptomatic surviving fish can be a source of infection.<\/jats:p>","DOI":"10.1111\/jfd.12996","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T07:14:36Z","timestamp":1554707676000},"page":"913-921","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Persistence of cyprinid herpesvirus 2 in asymptomatic goldfish <i>Carassius auratus<\/i> (L.) that survived an experimental infection"],"prefix":"10.1111","volume":"42","author":[{"given":"Chang","family":"Wei","sequence":"first","affiliation":[{"name":"Tokyo University of Marine Science and Technology Tokyo 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