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However, bacterial infections are affecting cleaner fish performance. <jats:italic>Vibrio anguillarum<\/jats:italic>, the aetiological agent of vibriosis, is one of the most frequent bacterial infections in lumpfish, and effective vaccine programmes against this pathogen have been identified as a high priority for lumpfish. Vibrogen\u20102 is a commercial polyvalent bath vaccine that contains formalin\u2010inactivated cultures of <jats:italic>V.\u00a0anguillarum<\/jats:italic> serotypes O1 and O2, and <jats:italic>Vibrio ordalii<\/jats:italic>. In this study, we evaluated Vibrogen\u20102 efficacy in lumpfish against a local isolated <jats:italic>V.\u00a0anguillarum<\/jats:italic> strain. Two groups of 125 lumpfish were bath\u2010immunized, bath\u2010boost\u2010immunized at four weeks post\u2010primary immunization, and intraperitoneally (i.p.) boost\u2010immunized at eight weeks post\u2010primary immunization. The control groups were i.p. mock\u2010immunized with PBS. Twenty\u2010seven weeks post\u2010primary immunization, the fish were i.p. challenged with 10 or 100 times the <jats:italic>V.\u00a0anguillarum<\/jats:italic> J360 LD<jats:sub>50<\/jats:sub> dose. After the challenge, survival was monitored daily, and samples of tissues were collected at ten days post\u2010challenge. Commercial vaccine Vibrogen\u20102 reduced <jats:italic>V.\u00a0anguillarum<\/jats:italic> tissue colonization and delayed mortality but did not confer immune protection to <jats:italic>C.\u00a0lumpus<\/jats:italic> against the <jats:italic>V.\u00a0anguillarum<\/jats:italic> i.p. challenge.<\/jats:p>","DOI":"10.1111\/jfd.13010","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T03:25:05Z","timestamp":1557804305000},"page":"1057-1064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Vibrogen\u20102 vaccine trial in lumpfish (<i>Cyclopterus lumpus<\/i>) against <i>Vibrio anguillarum<\/i>"],"prefix":"10.1111","volume":"42","author":[{"given":"Setu","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Faculty of Science Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trung","family":"Cao","sequence":"additional","affiliation":[{"name":"Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Faculty of Science Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Hossain","sequence":"additional","affiliation":[{"name":"Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Faculty of Science Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajarooba","family":"Gnanagobal","sequence":"additional","affiliation":[{"name":"Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Faculty of Science Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Vasquez","sequence":"additional","affiliation":[{"name":"Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Faculty of Science Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danny","family":"Boyce","sequence":"additional","affiliation":[{"name":"Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7701-3147","authenticated-orcid":false,"given":"Javier","family":"Santander","sequence":"additional","affiliation":[{"name":"Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Faculty of Science Memorial University of Newfoundland St John\u2019s Newfoundland Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2014.12.006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/691981"},{"key":"e_1_2_7_4_1","first-page":"447","volume-title":"Fish biologics: Serodiagnostics and vaccines","author":"Amend D.","year":"1981"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/f76-166"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00847.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00069-X"},{"key":"e_1_2_7_8_1","first-page":"17","article-title":"Wrasse as cleaner fish of farmed salmon","volume":"16","author":"Bjordal A.","year":"1991","journal-title":"Progress in Underwater Science"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/fsim.2001.0386"},{"key":"e_1_2_7_10_1","first-page":"451","volume-title":"Cleaner fish biology and aquaculture applications","author":"Boyce D.","year":"2018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(77)90019-9"},{"key":"e_1_2_7_12_1","unstructured":"Breiland M. 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