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In the present study, we have analysed the gene expression of extracellular matrix components and transcription factors involved in bone development in gilthead sea bream presenting different skeletal anomalies: lordosis (LD), lordosis\u2013scoliosis\u2013kyphosis (LSK) or opercular, dental or jaw malformations in comparison with control (CT) specimens. Results showed a possible link between the presence of LD and LSK and the significant downregulation of genes involved in osteoblasts' maturation and matrix mineralization (collagen type 1\u2010alpha, osteopontin, osteocalcin, matrix Gla protein and tissue non\u2010specific alkaline phosphatase), as well as in bone resorption (cathepsin K and matrix metalloproteinase 9) compared to CT animals. Contrarily, the key osteogenic transcription factor <jats:italic>runx2<\/jats:italic> was upregulated in the malformed vertebra suggesting impaired determination of mesenchymal stem cells towards the osteoblastic lineage. Despite the gene expression patterns of the other malformed structures were not affected in comparison with CT fish, the results of the present study may contribute in the long term to identify potential candidate gene profiles associated with column deformities that may help reducing the incidence of appearance of skeletal anomalies in this important aquaculture species.<\/jats:p>","DOI":"10.1111\/jfd.13019","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T10:14:57Z","timestamp":1560161697000},"page":"1169-1180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Gene expression analyses in malformed skeletal structures of gilthead sea bream (<i>Sparus aurata<\/i>)"],"prefix":"10.1111","volume":"42","author":[{"given":"Nat\u00e0lia","family":"Riera\u2010Heredia","sequence":"first","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Facultat de Biologia Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio J.","family":"V\u00e9lez","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Facultat de Biologia Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3126-2236","authenticated-orcid":false,"given":"Joaquim","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Facultat de Biologia Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2641-0361","authenticated-orcid":false,"given":"Isabel","family":"Navarro","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Facultat de Biologia Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0863-9728","authenticated-orcid":false,"given":"Encarnaci\u00f3n","family":"Capilla","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Facultat de Biologia Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,10]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Afonso J. 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