{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:15:09Z","timestamp":1787501709898,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T00:00:00Z","timestamp":1560211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007350","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["\u201cConvocatoria de Becas al Extranjero 2010 Segundo Periodo\""],"award-info":[{"award-number":["\u201cConvocatoria de Becas al Extranjero 2010 Segundo Periodo\""]}],"id":[{"id":"10.13039\/501100007350","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Francisella noatunensis<\/jats:italic>\n                    subsp.\n                    <jats:italic>orientalis<\/jats:italic>\n                    is a pathogen of tilapia and other warm\u2010water fish for which no vaccines are commercially available. In this study, a whole cell formalin\u2010inactivated vaccine was developed\u00a0for the first time using the highly virulent isolate STIR\u2010GUS\u2010F2f7 and the oil\u2010based adjuvant Montanide\u2122 ISA 763A VG. The efficacy of the vaccine was assessed in red Nile tilapia via intraperitoneal (i.p.) injection using homologous experimental infection and correlates of protection such as seral antibody production and bacterial loads in the spleen. For immunization, fish were i.p. injected with 0.1\u00a0ml of the vaccine, the adjuvant alone or PBS. At 840 degree days post\u2010vaccination, all fish were i.p. injected with 4.0\u00a0\u00d7\u00a010\n                    <jats:sup>3<\/jats:sup>\n                    CFU\/fish of pathogenic bacteria. The RPS at the end of the trial was 100% in the vaccinated group with significantly higher survival than in the adjuvant and control groups. The RPS in the adjuvant group was 42%, and no significant difference was seen in survival between this and the PBS group. Moreover, significantly higher antibody titres in the serum and significantly lower bacterial loads in the spleen were detected in the vaccinated fish by ELISA and qPCR, respectively. These findings highlight the potential of autogenous vaccines for controlling francisellosis in tilapia.\n                  <\/jats:p>","DOI":"10.1111\/jfd.13041","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T06:05:00Z","timestamp":1560233100000},"page":"1191-1200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Whole cell inactivated autogenous vaccine effectively protects red Nile tilapia (\n                    <i>Oreochromis niloticus<\/i>\n                    ) against francisellosis via intraperitoneal injection"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6260-5158","authenticated-orcid":false,"given":"Jos\u00e9 Gustavo","family":"Ram\u00edrez\u2010Paredes","sequence":"first","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Angel","family":"Mendoza\u2010Roldan","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0141-8113","authenticated-orcid":false,"given":"Benjamin","family":"Lopez\u2010Jimena","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid","family":"Shahin","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthijs","family":"Metselaar","sequence":"additional","affiliation":[{"name":"Benchmark Animal Health Ltd. Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3550-4842","authenticated-orcid":false,"given":"Kim D.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Aquaculture Research Group Moredun Research Institute Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Penman","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randolph H.","family":"Richards","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Adams","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences, Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-4648(95)80040-9"},{"key":"e_1_2_8_3_1","first-page":"447","article-title":"Potency testing of fish vaccines","volume":"49","author":"Amend D. 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