{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:40:34Z","timestamp":1788583234421,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T00:00:00Z","timestamp":1563321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Different antibiotic\u2010based drugs are being used for the treatment of <jats:italic>Aeromonas hydrophila<\/jats:italic> infection in rainbow trout, and several studies emphasize the use of medicinal plants as immunostimulants for prophylactic measure against Aeromoniasis disease. However, therapeutic effects of aqueous methanolic extracts of tetra (<jats:italic>Cotinus coggygria<\/jats:italic>) against <jats:italic>A.\u00a0hydrophila<\/jats:italic> in rainbow trout were not investigated. Four different concentrations of tetra extract (0 [control], 4, 8 and 12\u00a0mg\/100\u00a0\u00b5l) and also two different positive control groups (florfenicol and doxycycline antibiotics) were administered orally using feeding needles to individual rainbow trout, <jats:italic>Oncorhynchus mykiss<\/jats:italic> of all experimental groups twice a day after intramuscular inoculation of <jats:italic>A.\u00a0hydrophila<\/jats:italic>. The study period was for 10\u00a0days. On 0th, 3rd, 7th and 10th day, blood and tissues were collected from the fish and changes in humoral immune responses, haematology and immune\u2010related gene expressions were determined. In the study, superoxide radical production was decreased generally in all experimental groups except in 12\u00a0mg tetra and florfenicol treatments compared to control (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Lysozyme activity was generally decreased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), or no differences were observed in all experimental groups compared to the control. Myeloperoxidase activity was significantly increased in florfenicol\u2010treated fish group on 7th day (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Generally, myeloperoxidase activity showed an increase in almost all tetra\u2010treated groups. Haematological parameters increased but were not significantly high enough in treatments. Almost all immune\u2010related gene expressions were significantly enhanced on 3rd and 10th day of the study. Survival rate of 53.33% was found in control group. There were no significant differences in survival between control and 4\u00a0mg tetra\u2010treated group (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). All the other groups' survival rate was significantly increased compared to control. The highest survival rate was found in florfenicol group (80%). In 12\u00a0mg tetra\u2010, doxycycline\u2010 and 8\u00a0mg tetra\u2010treated groups, survival rate was recorded as 74.44%, 70% and 70%, respectively. Our results suggest that tetra methanolic extract is an effective therapeutic remedy against <jats:italic>A.\u00a0hydrophila<\/jats:italic> infection in rainbow trout at the dose of 24\u00a0mg\/32.34\u00a0g\u00a0body\u00a0weight\/day.<\/jats:p>","DOI":"10.1111\/jfd.13061","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T04:57:26Z","timestamp":1563425846000},"page":"1369-1381","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["A new potential therapeutic remedy against <i>Aeromonas hydrophila<\/i> infection in rainbow trout (<i>Oncorhynchus mykiss<\/i>) using tetra, <i>Cotinus coggygria<\/i>"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9459-8178","authenticated-orcid":false,"given":"Soner","family":"Bilen","sequence":"first","affiliation":[{"name":"Department of Basic Sciences, Faculty of Fisheries and Aquaculture Kastamonu University Kastamonu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Housam Taher Abdul Ghani","family":"Elbeshti","sequence":"additional","affiliation":[{"name":"Libyan Ministry of Marine Wealth Tripoli Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,17]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"185","article-title":"Histopathological alterations in estuarine catfish (Arius maculatus; Thunberg, 1792) due to Aeronomas hydrophila infection","volume":"1","author":"Alagappan K. M.","year":"2009","journal-title":"World Journal of Fish and Marine Sciences"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.07.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.06.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/life4020217"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2011.01271.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2016.08.040"},{"issue":"3","key":"e_1_2_9_8_1","first-page":"538","article-title":"The effects of oxygen supplementation on growth and survival of rainbow trout (Oncorhynchus mykiss) in different stocking densities","volume":"14","author":"Bilen S.","year":"2015","journal-title":"Iranian Journal of Fisheries Sciences"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3923\/javaa.2010.1275.1279"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.12.013"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.01.046"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2015.12.010"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4194\/1303-2712-v13_3_16"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.46989\/001c.20768","article-title":"Effects of dietary incorporation of tetra (Cotinus coggygria) extract on immune response and resistance to Aeromonas hydrophila in koi carp (Cyprinus carpio)","volume":"66","author":"Bilen S.","year":"2014","journal-title":"The Israeli Journal of Aquaculture\u2010Bamidgeh"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02852"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.12.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2018.1530458"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(02)00210-9"},{"issue":"5","key":"e_1_2_9_19_1","first-page":"4139","article-title":"Antimicrobial activity of Cotinus coggyria from Turkey","volume":"21","author":"Dulger B.","year":"2009","journal-title":"Asian Journal of Chemistry"},{"key":"e_1_2_9_20_1","first-page":"101","volume-title":"Techniques in fish immunology","author":"Ellis A.","year":"1990"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.13-327"},{"key":"e_1_2_9_22_1","volume-title":"Drugs approved for use in aquaculture","author":"Fda U.","year":"2007"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2008.12.023"},{"issue":"9","key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"3512","DOI":"10.1128\/iai.64.9.3512-3517.1996","article-title":"Involvement of superoxide and myeloperoxidase in oxygen\u2010dependent killing of Staphylococcus aureus by neutrophils","volume":"64","author":"Hampton M. B.","year":"1996","journal-title":"Infection and Immunity"},{"issue":"1","key":"e_1_2_9_25_1","first-page":"205","article-title":"Antibacterial activity of medicinal herbs against the fish pathogen Aeromonas hydrophila","volume":"8","author":"Haniffa M. A.","year":"2012","journal-title":"Journal of Agricultural Science and Technology"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/fsim.1998.0158"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.09.012"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.11.035"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2012.11.038"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(03)00022-X"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-013-0740-8"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.01.010"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.03.010"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.5530\/ijper.51.3s.13"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2006.07.004"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.05.005"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.02.042"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3923\/jbs.2010.800.804"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2008.07.008"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.03.022"},{"key":"e_1_2_9_42_1","first-page":"81","article-title":"Abuse of antibiotics in aquaculture and it's effects on human, aquatic animal and environment","volume":"2","author":"Rasul","year":"2017","journal-title":"Haya: the Saudi Journal of Life Sciences"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0426.2004.00606.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2008.04.009"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03854.x"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.08.003"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2004.00558.x"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)90062-0"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-7345.1996.tb00626.x"},{"issue":"33","key":"e_1_2_9_50_1","article-title":"Flagellin acting via TLR5 is the major\u2010activator of key signaling pathways leading to NF\u2010kappa B and proinflammatory gene program activation in intestinal epithelial cells","volume":"4","author":"Tallant T.","year":"2004","journal-title":"BMC Microbiology"},{"issue":"1","key":"e_1_2_9_51_1","first-page":"43","article-title":"Antimicrobial resistance profiles and tetracycline resistance genes of Escherichia coli in Mediterranean mussel and sea snails collected from Black Sea, Turkey","volume":"33","author":"Terzi E.","year":"2018","journal-title":"Alinteri Journal of Agriculture Science"},{"issue":"2","key":"e_1_2_9_52_1","first-page":"1594","article-title":"Antibiotic resistance genes of Escherichia coli in coastal marine environment of Eastern Black Sea, Turkey","volume":"28","author":"Terzi E.","year":"2019","journal-title":"Fresenius Environmental Bulletin"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.4194\/2459-1831-v2_2_03"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.4194\/trjfas.2009.0209"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2006.07.004"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1501\/Vetfak_0000002448"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2007.10.009"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2850210405"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13061","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13061","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T07:13:30Z","timestamp":1694330010000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,17]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1111\/jfd.13061"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13061","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,17]]},"assertion":[{"value":"2019-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}