{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:33:22Z","timestamp":1789659202427,"version":"4.0.1"},"reference-count":56,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T00:00:00Z","timestamp":1567468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A030313179"],"award-info":[{"award-number":["2017A030313179"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Nocardia seriolae<\/jats:italic> is the main pathogen responsible for fish nocardiosis. A mitochondrial\u2010targeting secretory protein (MTSP) 3141 with an N\u2010terminal transit peptide (TP) from <jats:italic>N. seriolae<\/jats:italic> was predicted by bioinformatic analysis based on the genomic sequence of the <jats:italic>N. seriolae<\/jats:italic> strain ZJ0503. However, the function of the MTSP3141 and its homologs remains totally unknown. In this study, mass spectrometry analysis of the extracellular products from <jats:italic>N. seriolae<\/jats:italic> proved that MTSP3141 was a secretory protein, subcellular localization research showed the MTSP3141\u2010GFP fusion protein co\u2010localized with mitochondria in fathead minnow (FHM) cells, the TP played an important role in mitochondria targeting, and only the TP located at N\u2010terminus but not C\u2010terminus can lead to mitochondria directing. Moreover, quantitative assays of mitochondrial membrane potential (\u0394\u03a8m) value, caspase\u20103 activity and apoptosis\u2010related gene (<jats:italic>Bcl<\/jats:italic>\u2010<jats:italic>2<\/jats:italic>, <jats:italic>Bax<\/jats:italic>, <jats:italic>Bad<\/jats:italic>, <jats:italic>Bid and p53<\/jats:italic>) mRNA expression suggested that cell apoptosis was induced in FHM cells by the overexpression of both MTSP3141 and MTSP3141\u0394TP (with the N\u2010terminal TP deleted) proteins. Taken together, the results of this study indicated that the MTSP3141 of <jats:italic>N. seriolae<\/jats:italic> was a secretory protein, might target mitochondria, induce apoptosis in host cells and function as a virulence factor.<\/jats:p>","DOI":"10.1111\/jfd.13062","type":"journal-article","created":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T05:40:49Z","timestamp":1567575649000},"page":"1493-1507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Identification of a mitochondrial\u2010targeting secretory protein from <i>Nocardia seriolae<\/i> which induces apoptosis in fathead minnow cells"],"prefix":"10.1111","volume":"42","author":[{"given":"Jianlin","family":"Chen","sequence":"first","affiliation":[{"name":"Shenzhen Institute of Guangdong Ocean University Shenzhen City China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals Fishery College of Guangdong Ocean University Zhanjiang City China"},{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6244-8060","authenticated-orcid":false,"given":"Liqun","family":"Xia","sequence":"additional","affiliation":[{"name":"Shenzhen Institute of Guangdong Ocean University Shenzhen City China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals Fishery College of Guangdong Ocean University Zhanjiang City China"},{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen City 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