{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:04:28Z","timestamp":1792008268508,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T00:00:00Z","timestamp":1568246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tilapia lake virus (TiLV)\u2010suspected samples of tilapia were collected from grow\u2010out ponds located with clinical signs and mortality ranged from 5% to 50%. The reverse transcription\u2013polymerase chain reaction (RT\u2010PCR) assay revealed the presence of TiLV in the disease outbreak ponds. Cell lines were developed from heart, gill and eye of Mozambique tilapia and characterized. Morphologically, these cell lines are composed of epithelioid cells. The optimum growth of these cells was observed at 28\u00b0C and 20% concentration of FBS. After cryopreservation, 70%\u201390% of cells were found to be viable. The cells of all three cell lines were found to be positive to fibronectin and pancytokeratin. PCR amplification of 16S rRNA and COI of <jats:italic>O.\u00a0mossambicus<\/jats:italic> confirmed the origin of these cell lines from <jats:italic>O.\u00a0mossambicus<\/jats:italic>. Heart and gill cell lines were found to be highly susceptible to TiLV and found to be useful for its isolation from infected fish samples. The experimental infection was carried out in <jats:italic>O.\u00a0niloticus<\/jats:italic> and <jats:italic>O.\u00a0mossambicus<\/jats:italic> using the TiLV propagated in susceptible cell lines. The RT\u2010PCR results revealed the presence of TiLV in brain, gill, liver, kidney, spleen, eye, muscle, intestine and heart of experimentally infected <jats:italic>O.\u00a0niloticus<\/jats:italic> and <jats:italic>O.\u00a0mossambicus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13075","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T01:20:26Z","timestamp":1568337626000},"page":"1543-1552","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["In vitro propagation of tilapia lake virus in cell lines developed from <i>Oreochromis mossambicus<\/i>"],"prefix":"10.1111","volume":"42","author":[{"given":"Ravi","family":"Nanthini","sequence":"first","affiliation":[{"name":"Aquatic Animal Health Laboratory C. 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