{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:30:50Z","timestamp":1788834650773,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"crossref","award":["MSD61I0034"],"award-info":[{"award-number":["MSD61I0034"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Petchra Pra Jom Klao Ph.D. Research Scholarship for International students from King Mongkut's University of Technology Thonburi (KMUTT), Thailand."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Detection of tilapia lake virus (TiLV) in tilapines is mainly from visceral organs of killed fish. However, lethal sampling might not be viable to broodstock and economically important ornamental cichlids. To contribute towards screening of the virus in asymptomatic infected fish, a subclinically infected population of Nile tilapia adults obtained from a local farm was preliminarily tested to compare different non\u2010lethal sampling methods, for example liver biopsy, gill biopsy, fin clip, mucus, faeces and blood for detection of TiLV. Only liver and blood samples gave positive results by PCR. Since blood sampling is relatively simpler, it was further used for five naturally co\u2010cultured juvenile fish species from above\u2010mentioned farm including 40 red tilapia broodstock and 20 Nile tilapia adults from two other different farms. The results showed that from the tested fish, 4 of 5 Nile tilapia, 2 of 5 hybrid red tilapia and 3 of 5 giant gourami blood samples tested positive, while 38 of 40 blood samples of red tilapia tested positive for TiLV in second\u2010step PCR. Sequencing representative PCR amplicons of positive samples confirmed sequence identity to TiLV. In conclusion, both blood and liver biopsy are practical non\u2010destructive sampling platforms for TiLV screening in cichlids with blood being more convenient, especially for tilapia broodstock.<\/jats:p>","DOI":"10.1111\/jfd.13076","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T00:39:55Z","timestamp":1570063195000},"page":"1629-1636","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Blood and liver biopsy for the non\u2010destructive screening of tilapia lake virus"],"prefix":"10.1111","volume":"42","author":[{"given":"Chutipong","family":"Chiamkunakorn","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Science King Mongkut's University of Technology Thonburi (KMUTT) Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4234-9406","authenticated-orcid":false,"given":"Vimbai 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