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We present a small\u2010scale transmission experiment with European otter and American mink fed with infected crayfish, and experiments testing survival of cultures of five <jats:italic>A.\u00a0astaci<\/jats:italic> strains at temperatures corresponding to those inside mammal and bird bodies. The pathogen was neither isolated from predator excrements nor transmitted to susceptible crayfish exposed to excrements. In agar\u2010based artificial media, it occasionally survived for 15\u00a0min at 40.5\u00b0C and for 45\u00a0min at 37.5\u00b0C, but not so when incubated at those temperatures for 45\u00a0min and 75\u00a0min, respectively. The five tested\u00a0strains differed in resistance to high temperatures, two (of genotype groups E and D) being more susceptible than other three (of groups A, B and D). Their survival to some extent varied when exposed to the same temperature after several weeks or months, suggesting that some yet\u2010unknown factors may influence <jats:italic>A.\u00a0astaci<\/jats:italic> resistance to temperature stress. Overall, we support the notion that passage through the digestive tract of warm\u2010blooded predators makes <jats:italic>A.\u00a0astaci<\/jats:italic> transmission unlikely.<\/jats:p>","DOI":"10.1111\/jfd.13109","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T01:39:20Z","timestamp":1573695560000},"page":"129-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Experimental evaluation of the potential for crayfish plague transmission through the digestive system of warm\u2010blooded predators"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8300-6701","authenticated-orcid":false,"given":"Ji\u0159\u00ed","family":"Svoboda","sequence":"first","affiliation":[{"name":"Department of Ecology Faculty of Science Charles University Prague Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Fischer","sequence":"additional","affiliation":[{"name":"Mining Museum P\u0159\u00edbram P\u0159\u00edbram Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Kozub\u00edkov\u00e1\u2010Balcarov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Ecology Faculty of Science Charles University Prague Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"\u0160\u0165\u00e1stkov\u00e1","sequence":"additional","affiliation":[{"name":"Gymn\u00e1zium Opatov Prague Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Br\u016f\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Czech Otter Foundation Fund T\u0159ebo\u0148 Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8118-8612","authenticated-orcid":false,"given":"Anton\u00edn","family":"Kouba","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries and Protection of Waters CENAKVA University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice Vod\u0148any Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-4370","authenticated-orcid":false,"given":"Adam","family":"Petrusek","sequence":"additional","affiliation":[{"name":"Department of Ecology Faculty of Science Charles University Prague Czechia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.limno.2014.07.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1982.tb00464.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.20506\/rst.15.2.943"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1986.tb01030.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1987.tb01086.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12898-019-0224-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2015.08.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02571"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03275"},{"key":"e_1_2_7_11_1","first-page":"131","article-title":"The crayfish plague fungus Aphanomyces astaci \u2013 Diagnosis, isolation, and pathobiology","volume":"7","author":"Cerenius L.","year":"1988","journal-title":"Freshwater Crayfish"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2435.2007.01341.x"},{"key":"e_1_2_7_13_1","first-page":"133","volume-title":"Atlas of crayfish in Europe","author":"Di\u00e9guez\u2010Uribeondo J.","year":"2006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.02.004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0953-7562(09)80716-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4098\/AT.arch.96-13"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2427.2002.00829.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070157"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.020"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2005.0413"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.12006"},{"key":"e_1_2_7_22_1","first-page":"71","article-title":"Spatial ecology and reproductive biology of an invasive American mink (Neovison vison) population \u2013 New findings from the Czech\u2010Moravian Highlands","volume":"57","author":"Hlav\u00e1\u010dov\u00e1 P.","year":"2014","journal-title":"Lutra"},{"key":"e_1_2_7_23_1","first-page":"49","volume-title":"Atlas of crayfish in Europe","author":"Holdich D. 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