{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:22:29Z","timestamp":1787494949640,"version":"build-2736575974"},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2017J01451"],"award-info":[{"award-number":["2017J01451"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    LuxR\u2010type transcriptional factors are essential in many bacterial physiological processes. However, there have been no reports on their roles in\n                    <jats:italic>Aeromonas hydrophila<\/jats:italic>\n                    . In this study, six stable silent strains were constructed using shRNA. Significant decreases in the expression levels of\n                    <jats:italic>\n                      luxR\n                      <jats:sub>05<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      luxR\n                      <jats:sub>08<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      luxR\n                      <jats:sub>19<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      luxR\n                      <jats:sub>11<\/jats:sub>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      luxR\n                      <jats:sub>164<\/jats:sub>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      luxR\n                      <jats:sub>165<\/jats:sub>\n                    <\/jats:italic>\n                    were shown in their respective strains by qRT\u2010PCR. The\n                    <jats:italic>\n                      luxR\n                      <jats:sub>05<\/jats:sub>\n                      \u2010RNAi\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      luxR\n                      <jats:sub>164<\/jats:sub>\n                      \u2010RNAi\n                    <\/jats:italic>\n                    exhibit the most significant changes in sensitivity to kanamycin and gentamicin. The\n                    <jats:italic>\n                      luxR\n                      <jats:sub>05<\/jats:sub>\n                    <\/jats:italic>\n                    \u2010RNAi showed minimum biofilm formation and the least motility, while\n                    <jats:italic>\n                      luxR\n                      <jats:sub>164<\/jats:sub>\n                      \u2010RNAi\n                    <\/jats:italic>\n                    showed minimum biofilm formation, adhesion, growth and extracellular protease activity compared to the wild\u2010type strain. In summary, the results of this paper suggest that all six\n                    <jats:italic>luxR<\/jats:italic>\n                    genes are involved in multiple physiological processes in\n                    <jats:italic>A.<\/jats:italic>\n                    \u00a0\n                    <jats:italic>hydrophila<\/jats:italic>\n                    and that the roles of\n                    <jats:italic>\n                      luxR\n                      <jats:sub>05<\/jats:sub>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      luxR\n                      <jats:sub>164<\/jats:sub>\n                    <\/jats:italic>\n                    are highly significant. The sensitivity of\n                    <jats:italic>\n                      luxR\n                      <jats:sub>05<\/jats:sub>\n                    <\/jats:italic>\n                    \u2010RNAi and\n                    <jats:italic>\n                      luxR\n                      <jats:sub>164<\/jats:sub>\n                    <\/jats:italic>\n                    \u2010RNAi to drugs may be closely related to biofilm formation. The\n                    <jats:italic>\n                      luxR\n                      <jats:sub>05<\/jats:sub>\n                    <\/jats:italic>\n                    may play an important role in the pathogenicity of\n                    <jats:italic>A.\u00a0hydrophila<\/jats:italic>\n                    by regulating the movement, adhesion and biofilm formation of bacteria, whereas\n                    <jats:italic>\n                      luxR\n                      <jats:sub>164<\/jats:sub>\n                    <\/jats:italic>\n                    may be involved in similar functions by regulating bacterial adhesion, extracellular enzyme activity and growth. These results help further our understanding of the drug resistance and pathogenesis of\n                    <jats:italic>A.\u00a0hydrophila<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/jfd.13114","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T18:59:22Z","timestamp":1574794762000},"page":"215-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Role of LuxR\u2010type regulators in fish pathogenic\n                    <i>Aeromonas hydrophila<\/i>"],"prefix":"10.1111","volume":"43","author":[{"given":"Leilei","family":"Mao","sequence":"first","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7850-7010","authenticated-orcid":false,"given":"Yingxue","family":"Qin","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"},{"name":"Fujian Fisheries Technology Extension Center Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Kang","sequence":"additional","affiliation":[{"name":"Fujian Fisheries Technology Extension Center Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wu","sequence":"additional","affiliation":[{"name":"Fujian Fisheries Technology Extension Center Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixing","family":"Huang","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8698-3121","authenticated-orcid":false,"given":"Qingpi","family":"Yan","sequence":"additional","affiliation":[{"name":"Fisheries College Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Jimei University Xiamen China"},{"name":"Fujian Fisheries Technology Extension Center Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.12.033"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"232","article-title":"Pathogenicity of Aeromonas hydrophila in giant freshwater prawn Macrobrachium rosenbergii, cultured in East Malaysia","volume":"14","author":"Abdolnabi S.","year":"2015","journal-title":"Iranian Journal of Fisheries Sciences"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04054.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46569-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00144-10"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1992.tb00653.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638717708584"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3233-3241.2000"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.10.032"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5385-5394.2001"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4272-4280.2005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23219"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5427-5433.1999"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2005.02.009"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2013.06.056"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2003.10.003"},{"issue":"2","key":"e_1_2_9_19_1","first-page":"174","article-title":"Molecular mechanisms of quinolone resistance in Aeromonas hydrophilia.","volume":"54","author":"Fang Y.","year":"2014","journal-title":"Acta Microbiologica Sinica"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2026.1993.tb00114.x"},{"key":"e_1_2_9_21_1","volume-title":"The pathogenic bacteria identification and correlative immune studies in eels","author":"Guo S. L.","year":"2006"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-015-0432-9"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00207"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.04.031"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.517"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12957"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2017.03.002"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.468"},{"issue":"1","key":"e_1_2_9_29_1","first-page":"31","article-title":"Multiple drug resistance of Aeromonas hydrophila isolates from chicken samples collected from Mhow and Indore city of Madhyapradesh","volume":"2","author":"Kaskhedikar M.","year":"2009","journal-title":"Veterinary World"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00561-13"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx165"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2018.01.023"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-017-2204-y"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.451"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12848"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.511"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1139\/W07-085"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13001"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.17.3.477"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2009.00646.x"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-018-2453-4"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000175714.68624.74"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.04.008"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.05.016"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.07.006"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.48.4.865-867.1984"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01185.x"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.05.004"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1924-1935.2002"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1557\/mrs.2011.67"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00037-3"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00314-08"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(02)00009-0"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12991"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.07.011"},{"issue":"10","key":"e_1_2_9_57_1","first-page":"1435","article-title":"Effect of Klebsiella pneumoniae KbvR regulator on bacterial biofilm formation and capsular synthesis","volume":"36","author":"Xu L.","year":"2016","journal-title":"Journal of Southern Medical University"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23262"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.06.051"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.03.021"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.06.029"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179549"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000718"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13114","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13114","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T13:34:29Z","timestamp":1694093669000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,26]]},"references-count":62,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1111\/jfd.13114"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13114","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,26]]},"assertion":[{"value":"2019-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}