{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:33:11Z","timestamp":1789659191767,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T00:00:00Z","timestamp":1584662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A030313179"],"award-info":[{"award-number":["2017A030313179"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Nocardia seriolae<\/jats:italic>, a Gram\u2010positive bacterium, is the main pathogen of fish nocardiosis. Protein NlpC\/P60 is a cell\u2010wall peptidase and a potential virulence factor of <jats:italic>N. seriolae<\/jats:italic>. Subcellular localization research revealed that both NlpC\/P60\u2010GFP and NlpC\/P60\u0394sig\u2010GFP fusion proteins were evenly distributed in the whole cell of fathead minnow (FHM) cells. Furthermore, typical apoptotic features, such as nuclear pyrosis and apoptotic bodies, were observed in the transfected FHM cells and grouper spleen cells by the overexpression of protein NlpC\/P60. Then, quantitative assays of mitochondrial membrane potential (\u0394\u03a8m) value, caspase\u20103 activity and apoptosis\u2010related gene (<jats:italic>Bax, BNIP3, TNF1<\/jats:italic> and <jats:italic>TNF6<\/jats:italic>) mRNA expression were conducted. The results showed that \u0394\u03a8m was decreased, caspase\u20103 was significantly activated, and the mRNA expression of pro\u2010apoptotic genes (<jats:italic>Bax<\/jats:italic> and <jats:italic>BNIP3<\/jats:italic>) and tumour necrosis factors (<jats:italic>TNF1<\/jats:italic> and <jats:italic>TNF6<\/jats:italic>) was up\u2010regulated in <jats:italic>NlpC\/P60<\/jats:italic>\u2010overexpressed cells. Taken together, the results indicated that the protein NlpC\/P60 of <jats:italic>N. seriolae<\/jats:italic> might involve in apoptosis regulation. This study may lay the foundation for further study on the function of <jats:italic>N. seriolae<\/jats:italic> NlpC\/P60 and promote the understanding of the virulence factors and pathogenic mechanism of <jats:italic>N. seriolae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13154","type":"journal-article","created":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T18:26:14Z","timestamp":1584728774000},"page":"571-581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification of a cell\u2010wall peptidase (NlpC\/P60) from <i>Nocardia seriolae<\/i> which induces apoptosis in fathead minnow cells"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5443-980X","authenticated-orcid":false,"given":"Suying","family":"Hou","sequence":"first","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of Fisheries Guangdong Ocean University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoquan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of Fisheries Guangdong Ocean University Zhanjiang 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Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of Fisheries Guangdong Ocean University Zhanjiang China"},{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry Guangxi Beibu Gulf Marine Research Center Guangxi Academy of Sciences Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of Fisheries Guangdong Ocean University Zhanjiang 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