{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:30:51Z","timestamp":1788834651329,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The severe mortality of fish due to the infection of megalocytivirus caused significant economic losses. Since 2011, megalocytivirus (giant gourami iridovirus (GGIV)) has become the main pathogen in giant gourami (<jats:italic>Osphronemus goramy<\/jats:italic>), particularly in West Java, Central Java and Bali. This study aimed to develop primary cell culture from spleen as the target organ for propagating megalocytivirus in vitro, which was developed by explant method with enzymatic dissociation. Optimization was carried out at incubation temperature, medium and serum concentrations. The origin of the primary cell, cell susceptibility and GGIV pathogenicity were observed. The results showed that the primary cell (GP cells) can grow well in 10% foetal bovine serum L\u201015 medium at 27\u00b0C, which was sufficient for cell growth. PCR and BLAST analyses showed the primary cell was originated from giant gourami. In infected GP cells, cell enlargement and cell rounding were observed. Virus propagated in GP cells was highly virulent when injecting giant gourami in an artificial infection experiment. Intraperitoneal injection of diluted virus supernatant showed 100% mortality in 7\u201311 days post\u2010injection and 97% mortality in 21\u00a0days post\u2010cohabitation, with abnormalities observed in spleen and kidney. In conclusion, GP cell was successfully subcultured for more than 30 passages and susceptible to GGIV.<\/jats:p>","DOI":"10.1111\/jfd.13155","type":"journal-article","created":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T23:56:11Z","timestamp":1591660571000},"page":"829-838","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of primary cell culture from spleen of giant gourami <i>Osphronemus goramy<\/i> for propagation of giant gourami iridovirus (GGIV)"],"prefix":"10.1111","volume":"43","author":[{"given":"Lila","family":"Gardenia","sequence":"first","affiliation":[{"name":"Government of Indonesia Ministry of Marine Affairs and Fisheries Research Institute for Freshwater Aquaculture and Fisheries Extension Bogor Indonesia"},{"name":"Department of Aquaculture Faculty of Fisheries and Marine Science IPB University (Bogor Agricultural University) Bogor Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-8329","authenticated-orcid":false,"given":"Sukenda","family":"Sukenda","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Faculty of Fisheries and Marine Science IPB University (Bogor Agricultural University) Bogor Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Zairin","family":"Junior","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Faculty of Fisheries and Marine Science IPB University (Bogor Agricultural University) Bogor Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Lusiastuti","sequence":"additional","affiliation":[{"name":"Government of Indonesia Ministry of Marine Affairs and Fisheries Research Institute for Freshwater Aquaculture and Fisheries Extension Bogor Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alimuddin","family":"Alimuddin","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Faculty of Fisheries and Marine Science IPB University (Bogor Agricultural University) Bogor Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"issue":"24","key":"e_1_2_8_2_1","first-page":"7806","article-title":"Growth control of human mammary cancer cells (MCF\u20107 cells) in culture: Effect of estradiol and growth factors in serum\u2010containing medium","volume":"50","author":"Aakvaag A.","year":"1990","journal-title":"Cancer Research"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1290\/1543-706X(2003)039<0291:FOAPCC>2.0.CO;2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2005.10.012"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao058127"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-09-0315"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao060241"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.1999.00152.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.01.026"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.10.016"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.04.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471747598"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.12540"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(03)00010-0"},{"key":"e_1_2_8_15_1","first-page":"155","article-title":"Serum and growth factors in cell culture media\u2010An introductory review","volume":"66","author":"Griffiths J. 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