{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:47:49Z","timestamp":1791582469606,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T00:00:00Z","timestamp":1584662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries in Korea","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previously, <jats:italic>Aeromonas sobria<\/jats:italic> and <jats:italic>A.\u00a0salmonicida<\/jats:italic> were identified to be the most prevalent species in salmonid farms in Korea. In this study, we evaluated the biochemical characteristics, antibiotic susceptibility and pathogenicity of <jats:italic>A.\u00a0salmonicida<\/jats:italic> (3 isolates) and <jats:italic>A.\u00a0sobria<\/jats:italic> (8 isolates) isolated from salmonids, and further investigated efficacy of <jats:italic>A.\u00a0salmonicida<\/jats:italic> vaccine. In antibiotic susceptibility test, all of <jats:italic>A.\u00a0sobria<\/jats:italic> isolates were resistant to amoxicillin and ampicillin. Six <jats:italic>A.\u00a0sobria<\/jats:italic> and two <jats:italic>A.\u00a0salmonicida<\/jats:italic> isolates were resistant to oxytetracycline. In challenge test, <jats:italic>A.\u00a0sobria<\/jats:italic> isolates exhibited low pathogenicity in rainbow trout (<jats:italic>Oncorhynchus mykiss<\/jats:italic>) while one <jats:italic>A.\u00a0salmonicida<\/jats:italic> isolate showed high pathogenicity with LD<jats:sub>50<\/jats:sub> of 6.4\u00a0\u00d7\u00a010<jats:sup>3<\/jats:sup>\u00a0CFU\/fish in rainbow trout and coho salmon (<jats:italic>Oncorhynchus kisutch<\/jats:italic>). Among virulence factors, secretion apparatus (ascV and ascC) and transcription regulatory protein (exsA) of type 3 secretion system and A\u2010layer protein genes were differentially detected in DNA or cDNA of <jats:italic>A.\u00a0salmonicida<\/jats:italic> isolates, indicating their contribution to the pathogenicity. A formalin\u2010killed vaccine of highly pathogenic <jats:italic>A.\u00a0salmonicida<\/jats:italic> isolate exhibited a protective effect with relative survival rate of 81.8% and 82.9% at 8\u00a0weeks and 16\u00a0weeks post\u2010vaccination, respectively, in challenge test.<\/jats:p>","DOI":"10.1111\/jfd.13158","type":"journal-article","created":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T18:24:56Z","timestamp":1584728696000},"page":"609-620","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Characterization of <i>Aeromonas salmonicida<\/i> and <i>A.\u00a0sobria<\/i> isolated from cultured salmonid fish in Korea and development of a vaccine against furunculosis"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8558-1438","authenticated-orcid":false,"given":"Jongwon","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Marine Biotechnology Gangneung\u2010Wonju National University Gangneung Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhee","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Marine Biotechnology Gangneung\u2010Wonju National University Gangneung Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2348-2357.2003"},{"key":"e_1_2_8_3_1","first-page":"215","article-title":"Glucose repression of pigment production in atypical isolates of Aeromonas salmonicida responsible for goldfish ulcer disease","volume":"72","author":"Altmann K.","year":"1992","journal-title":"Microbios"},{"key":"e_1_2_8_4_1","first-page":"447","article-title":"Potency testing of fish vaccines","author":"Amend D. 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