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It is well known that betanodaviruses are neurotropic viruses that replicate in nerve tissues, preferentially brain and retina. However, routes of entry and progression of the virus in the central nervous system (CNS) remain unclear. The role of four tissues\u2014eye, oesophagus, gills and skin\u2014as possible gateways of a betanodavirus, the <jats:italic>redspotted grouper nervous necrosis virus<\/jats:italic> (RGNNV), was investigated after experimental challenges performed on European seabass juveniles. The dispersal pattern of Betanodavirus at primarily stages of the disease was also assessed, using a real\u2010time qPCR assay. The development of typical clinical signs of VER, the presence of characteristic histopathological lesions in the brain and retina and the detection of viral RNA in the tissues of all experimental groups ascertained that successful invasion of RGNNV under all experimental routes was achieved. Transneuronal spread along pathways known to be connected to the initial site of entry seems to be the predominant scenario of viral progression in the CNS. Furthermore, viraemia appeared only after the installation of the infection in the brain.<\/jats:p>","DOI":"10.1111\/jfd.13215","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T23:43:08Z","timestamp":1599176588000},"page":"1363-1371","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Investigation of routes of entry and dispersal pattern of RGNNV in tissues of European sea bass, <i>Dicentrarchus labrax<\/i>"],"prefix":"10.1111","volume":"43","author":[{"given":"Eirini","family":"Lampou","sequence":"first","affiliation":[{"name":"Laboratory of Ichthyology and Aquatic Animal Medicine Faculty of Veterinary Medicine University of Thessaly  karditsa Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrysostomos","family":"Dovas","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory School of Veterinary Medicine Faculty of Health Sciences Aristotle 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