{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:35:29Z","timestamp":1780472129809,"version":"3.54.1"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["107\u20102313\u2010B\u2010020\u2010012\u2010MY3"],"award-info":[{"award-number":["107\u20102313\u2010B\u2010020\u2010012\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Francisella orientalis<\/jats:italic> is a highly virulent, emerging bacterium that causes mass mortalities in tilapia. This pathogen also affects numerous other warm\u2010water fish species, including three\u2010line grunt, hybrid striped bass and various ornamental fish. This study sheds light on two new species of fish that are susceptible to <jats:italic>F.\u00a0orientalis<\/jats:italic>. Asian seabass and largemouth bass showed variable levels of susceptibility in a bacterial challenge experiment. After intraperitoneally injected with a dose of 10<jats:sup>6<\/jats:sup>\u00a0CFU\/fish, a total of 64.28% and 21.42% mortalities were obtained in Asian seabass and largemouth bass, respectively. Meanwhile, Nile tilapia showed acute mortality of 100%. All fish showed typical lesions of francisellosis, including multifocal granulomas in the spleen and head kidney. Immunohistochemical analysis revealed strong positive signals inside the granulomas of all fish. The bacterial recovery in solid media from infected fish was highest in Nile tilapia (85.71%), followed by Asian seabass (35.71%) and largemouth bass (21.42%). PCR results tested 100% positive for Nile tilapia, and 78.57% and 21.42% for Asian seabass and largemouth bass, respectively. In conclusion, Asian seabass and largemouth bass are susceptible to this pathogen, which warrants new management strategies when employing predation polyculture systems of these species with tilapia.<\/jats:p>","DOI":"10.1111\/jfd.13217","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T07:45:45Z","timestamp":1595490345000},"page":"1097-1106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of the pathogenicity of <i>Francisella orientalis<\/i> in Nile tilapia (<i>Oreochromis niloticus<\/i>), Asian seabass (<i>Lates calcarifer<\/i>) and largemouth bass (<i>Micropterus salmoides<\/i>) through experimental intraperitoneal infection"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2314-2031","authenticated-orcid":false,"given":"Sayuj","family":"Poudyal","sequence":"first","affiliation":[{"name":"International Degree Program of Ornamental Fish Technology and Aquatic Animal Health International College National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theeraporn","family":"Pulpipat","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Chi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Southern Taiwan Fish Diseases Research Center College of Veterinary Medicine National Pingtung University of Science and Technology  Pingtung Taiwan"},{"name":"Research Center for Fish Vaccine and Diseases College of Veterinary Medicine National Pingtung University of Science and 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