{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T09:30:43Z","timestamp":1768642243930,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The bunyavirus Mourilyan virus (MoV) occurs commonly in Black tiger (<jats:italic>Penaeus monodon<\/jats:italic>) and kuruma shrimp (<jats:italic>Penaeus\u00a0japonicus<\/jats:italic>) farmed in eastern Australia. There is circumstantial evidence of MoV causing mortalities among <jats:italic>P.\u00a0japonicus<\/jats:italic> moved from farm ponds to tanks for rearing as broodstock. To directly assess its pathogenic potential, independent cohorts of pond\u2010 (<jats:italic>n<\/jats:italic>\u00a0=\u00a024) or tank\u2010reared juvenile (<jats:italic>n<\/jats:italic>\u00a0=\u00a021) <jats:italic>P.\u00a0japonicus<\/jats:italic> were challenged intramuscularly with a cephalothorax tissue homogenate of <jats:italic>P.\u00a0monodon<\/jats:italic> containing high loads of MoV (1.48\u00a0\u00b1\u00a00.28\u00a0\u00d7\u00a010<jats:sup>8<\/jats:sup> MoV RNA copies\/\u00b5g total RNA). In each trial, mortalities accumulated gradually among the saline\u2010injected controls. Mortality onset occurred 12\u201314\u00a0days earlier in the pond\u2010reared shrimp, possibly due to them possessing low\u2010level pre\u2010existing MoV infections. Despite the time to onset of mortality differing, Kaplan\u2013Meier survival analyses confirmed mortality rates to be significantly higher in both the pond\u2010 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.017) and tank\u2010reared shrimp <jats:italic>(p<\/jats:italic>\u00a0=\u00a0.031) challenged with MoV. RT\u2010qPCR data on shrimp sampled progressively over each trial showed high loads of MoV to establish following challenge and discounted GAV and other endemic viruses from contributing to mortality. Together, the data show that acute MoV infection can adversely compromise the survival of juvenile <jats:italic>P.\u00a0japonicus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13244","type":"journal-article","created":{"date-parts":[[2020,10,10]],"date-time":"2020-10-10T18:42:17Z","timestamp":1602355337000},"page":"1401-1407","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mourilyan virus pathogenicity in kuruma shrimp (<i>Penaeus japonicus<\/i>)"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0454-4825","authenticated-orcid":false,"given":"Jeff A.","family":"Cowley","sequence":"first","affiliation":[{"name":"Livestock &amp; Aquaculture CSIRO Agriculture &amp; Food Queensland Bioscience Precinct St. Lucia QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-019-04253-6"},{"issue":"2","key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3354\/dao040085","article-title":"The haemocytic origin of lymphoid organ spheroid cells in the penaeid prawn Penaeus monodon","volume":"40","author":"Anggraeni M. 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