{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T17:19:59Z","timestamp":1770225599759,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptococcus agalactiae<\/jats:italic> is a Gram\u2010positive facultative intracellular bacterium that leads to severe economic loss of tilapia worldwide. Previous studies demonstrated that CD40 contributes to host protection against intracellular injection. In this study, CD40 was characterized from Nile tilapia (<jats:italic>Oreochromis niloticus<\/jats:italic>), named OnCD40. Sequence analysis showed that open reading frame of OnCD40 was 933\u00a0bp, containing a single peptide, a transmembrane domain and four cysteine\u2010rich domains. The qRT\u2010PCR revealed that OnCD40 was expressed in all examined tissues with the most abundant ones in spleen and thymus. After <jats:italic>S. agalactiae<\/jats:italic> stimulation, the expression of OnCD40 was significantly induced in most of the detected organs. Moreover, OnCD40\u2010overexpressing fish elicited significant protection against subsequent <jats:italic>S. agalactiae<\/jats:italic> challenge; approximately 10000\u2010fold fewer bacteria were detected in spleen of OnCD40\u2010overexpressing fish in comparison with control fish. Thus, CD40 had protecting function in Nile tilapia against intracellular pathogens.<\/jats:p>","DOI":"10.1111\/jfd.13266","type":"journal-article","created":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T04:08:50Z","timestamp":1600747730000},"page":"45-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["CD40 induces an antimicrobial response against the intracellular pathogen <i>Streptococcus agalactiae<\/i> in Nile tilapia, <i>Oreochromis niloticus<\/i>"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0155-8079","authenticated-orcid":false,"given":"Hongli","family":"Xia","sequence":"first","affiliation":[{"name":"Shenzhen Institute of Guangdong Ocean University Shenzhen Guangdong China"},{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen 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