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In this study, a novel galectin\u2010related protein B (<jats:italic>OnGRPB<\/jats:italic>) was identified from Nile tilapia (<jats:italic>Oreochromis niloticus<\/jats:italic>). The open reading frame of <jats:italic>OnGRPB<\/jats:italic> was 438\u00a0bp and encoded a peptide of 145 amino acids. The deduced protein sequence of OnGRPB possessed a conserved carbohydrate recognition domain (CRD) with partial sugar binding sites (N\u2010R, V\u2010N and W\u2010E) and shared high identities with other fish GRPB proteins. The qRT\u2010PCR analysis found that <jats:italic>OnGRPB<\/jats:italic> was widely distributed in various tissues and monocyte\/macrophages (Mo\/M\u03a6) of healthy tilapia. After <jats:italic>Streptococcus agalactiae<\/jats:italic> infection, <jats:italic>OnGRPB<\/jats:italic> transcripts were significantly up\u2010regulated in liver, spleen, head kidney and Mo\/M\u03a6. The recombinant OnGRPB protein (rOnGRPB) had the binding activity and agglutination ability to bacteria. Also, rOnGRPB could modulate antibacterial activity and inflammatory factor expression of Mo\/M\u03a6. These data collectively indicate that OnGRPB plays roles in the immune response of Nile tilapia against bacterial infection.<\/jats:p>","DOI":"10.1111\/jfd.13273","type":"journal-article","created":{"date-parts":[[2020,10,11]],"date-time":"2020-10-11T15:45:44Z","timestamp":1602431144000},"page":"171-180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization and functional analysis of a galectin\u2010related protein B from Nile tilapia involved in the immune response to bacterial infection"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1595-9543","authenticated-orcid":false,"given":"Jinzhong","family":"Niu","sequence":"first","affiliation":[{"name":"College of Fishery Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal Key Laboratory of 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