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This work investigated the stress effect on the attachment of <jats:italic>L. salmonis<\/jats:italic> copepodids to Atlantic salmon through two approaches: (a) handling by netting and air exposure (acute stress), and (b) crowding with restricted surface access in a tank (chronic stress). In the first experiment, we compared the number of attached <jats:italic>L. salmonis<\/jats:italic> and cortisol levels between a group of handled salmon and a control group. In the second experiment, a group of densely packed salmon was compared to a control group based on the number of attached copepodids, cortisol levels and neutrophil:lymphocyte ratios. Handled salmon showed significantly higher plasma cortisol levels (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001) and more attached copepodids (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.01) than control salmon. Conversely, the cortisol level and number of attached copepodids were not significantly different between the densely packed and control salmon (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The neutrophil:lymphocyte ratio was significantly higher (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0014) in the densely packed salmon than in the control salmon. Handling salmon increased their risk of infestation by <jats:italic>L. salmonis<\/jats:italic>. This has implications for reinfestation rates following delousing treatments in commercial salmon aquaculture, which often involve crowding and handling salmon.<\/jats:p>","DOI":"10.1111\/jfd.13286","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T16:24:37Z","timestamp":1603902277000},"page":"327-336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effect of handling and crowding on the susceptibility of Atlantic salmon (<i>Salmo salar<\/i> L.) to <i>Lepeophtheirus salmonis<\/i> (Kr\u00f8yer) copepodids"],"prefix":"10.1111","volume":"44","author":[{"given":"Cyril","family":"Delfosse","sequence":"first","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"},{"name":"IRSEA\u2010ARC Daugstad Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0127-8587","authenticated-orcid":false,"given":"Patrick","family":"Pageat","sequence":"additional","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"},{"name":"IRSEA\u2010ARC Daugstad Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Lafont\u2010Lecuelle","sequence":"additional","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-1146","authenticated-orcid":false,"given":"Pietro","family":"Asproni","sequence":"additional","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Chabaud","sequence":"additional","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"},{"name":"IRSEA\u2010ARC Daugstad Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Cozzi","sequence":"additional","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"},{"name":"IRSEA\u2010ARC Daugstad Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7230-4471","authenticated-orcid":false,"given":"C\u00e9cile","family":"Bienboire\u2010Frosini","sequence":"additional","affiliation":[{"name":"Research Institute in Semiochemistry and Applied Ethology (IRSEA) Apt France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00134"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2014.12.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/691981"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.applanim.2006.09.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1139\/f05-231"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2004.03.026"},{"key":"e_1_2_9_8_1","first-page":"1","article-title":"Prevention not cure: A review of methods to avoid sea lice infestations in salmon aquaculture","author":"Barrett L. 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