{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T15:14:05Z","timestamp":1782832445954,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glutamic endopeptidases (Glu), belonging to the class of serine proteases, are a subfamily of chymotrypsin\u2010like proteolytic enzymes, which are regarded as important virulence factors in bacteria. However, the roles of glutamic endopeptidases of <jats:italic>Nocardia seriolae<\/jats:italic> in pathogenic process still remain uncertain. Here, a glutamic endopeptidase homolog from <jats:italic>N. seriolae<\/jats:italic> (GluNS) was cloned and its function was elucidated. <jats:italic>GluNS<\/jats:italic> encoded a 414\u2010aa protein which shared 93% identity to <jats:italic>N. concava<\/jats:italic>. In the phylogenetic tree, the glutamic endopeptidases of genus Nocardia clustered together firstly and then clustered with Streptomyces species. Moreover, GluNS was identified to be a secreted protein of <jats:italic>N. seriolae<\/jats:italic> and localized in the mitochondria of FHM cells. The transient overexpression of GluNS significantly induced increase in caspase\u20103 activity and decrease in \u0394\u03a8m values in FHM cells. The number of apoptotic bodies was remarkably higher than that in control group. Taken together, GluNS overexpression induced apoptotic characteristics in FHM cells. This study provided new insights into the function of glutamic endopeptidase from <jats:italic>N. seriolae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfd.13311","type":"journal-article","created":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T18:01:11Z","timestamp":1606500071000},"page":"813-821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization and function study of a glutamyl endopeptidase homolog from <i>Nocardia seriolae<\/i>"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1662-2130","authenticated-orcid":false,"given":"Wenji","family":"Wang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals College of Fisheries Guangdong Ocean University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suying","family":"Hou","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals College of Fisheries Guangdong Ocean University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoquan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals College of Fisheries Guangdong Ocean University Zhanjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6244-8060","authenticated-orcid":false,"given":"Liqun","family":"Xia","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings Shenzhen Institute of Guangdong Ocean University Shenzhen China"},{"name":"Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals College of Fisheries Guangdong Ocean University Zhanjiang China"},{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry Guangxi Beibu Gulf Marine 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