{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:27:55Z","timestamp":1760956075328,"version":"3.40.5"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T00:00:00Z","timestamp":1607040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Atypical <jats:italic>Aeromonas salmonicida<\/jats:italic> (a<jats:italic>As<\/jats:italic>) is currently one of the most routinely recovered bacterial pathogens isolated during disease outbreaks in farmed cleaner fish, ballan wrasse (<jats:italic>Labrus bergylta<\/jats:italic>, Ascanius). Vibrionaceae family bacteria have also been isolated from ballan wrasse in Scotland. This study determined the infectivity, pathogenicity and virulence of a<jats:italic>As<\/jats:italic> and Vibrionaceae isolates in juvenile farmed ballan wrasse (<jats:italic>n<\/jats:italic>\u00a0=\u00a050; approx. 2\u00a0g) using a bath challenge, and fish were monitored for a period of 16\u00a0days. Atypical <jats:italic>As<\/jats:italic> caused significant mortalities in contrast to Vibrionaceae isolates. Notably, differential virulence was observed between two a<jats:italic>As vapA<\/jats:italic> type V strains at similar challenge doses. Diseased fish exhibited a systemic infection where a<jats:italic>As<\/jats:italic> was detected in all analysed tissues (liver, spleen and kidney) by PCR and qPCR. Macroscopically, moribund and survivor fish exhibited hepatomegaly and splenomegaly. In moribund and surviving fish, histopathology showed granulomatous hepatitis with eosinophilic granular cells surrounding bacterial colonies and endocarditis along with splenic histiocytosis. This is the first report of a successful a<jats:italic>As<\/jats:italic> bath challenge model for juvenile ballan wrasse which provides an important tool for future studies on vaccine efficacy and immunocompetence.<\/jats:p>","DOI":"10.1111\/jfd.13312","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T07:50:31Z","timestamp":1607154631000},"page":"823-835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Novel atypical <i>Aeromonas salmonicida<\/i> bath challenge model for juvenile ballan wrasse (<i>Labrus bergylta,<\/i> Ascanius)"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7956-9627","authenticated-orcid":false,"given":"Athina","family":"Papadopoulou","sequence":"first","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn","family":"Garvey","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Hill","sequence":"additional","affiliation":[{"name":"Centre for Environment, Fisheries and Aquaculture Science Weymouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6260-5158","authenticated-orcid":false,"given":"Jose G.","family":"Ramirez\u2010Paredes","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"},{"name":"Ridgeway Biologicals Ltd. a Ceva Sant\u00e9 Animale company Compton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean J.","family":"Monaghan","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna L.","family":"Baily","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Davie","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioanna","family":"Katsiadaki","sequence":"additional","affiliation":[{"name":"Centre for Environment, Fisheries and Aquaculture Science Weymouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6283-6028","authenticated-orcid":false,"given":"David","family":"Verner\u2010Jeffreys","sequence":"additional","affiliation":[{"name":"Centre for Environment, Fisheries and Aquaculture Science Weymouth UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Wallis","sequence":"additional","affiliation":[{"name":"Ridgeway Biologicals Ltd. a Ceva Sant\u00e9 Animale company Compton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herve","family":"Migaud","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture Faculty of Natural Sciences University of Stirling Stirling UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.04.066"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8640(1994)056<0258:DOPAAS>2.3.CO;2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-32674-0_5"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(89)80026-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12450"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.104966"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12290"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00001-17"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01643.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2014.04.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2014.02.038"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.08.006"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1996.0075"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1981.tb01143.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2016.03.024"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02342"},{"issue":"7","key":"e_1_2_8_18_1","first-page":"fnz074","article-title":"Biogeography of the fish pathogen Aeromonas salmonicida inferred by vapA genotyping","volume":"366","author":"Gulla S.","year":"2019","journal-title":"FEMS Microbiology Letters"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12420"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12367"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2010.01217.x"},{"volume-title":"The salmon lice Lepeophtheirus salmonis as the cause of disease in farmed salmonids","year":"1976","author":"Hastein T.","key":"e_1_2_8_22_1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.41"},{"volume-title":"Beste praksis for medikamentfrie metoder for lakseluskontroll (MEDFRI)\u2010Faglig sluttrapport","year":"2017","author":"Holan A. B.","key":"e_1_2_8_24_1"},{"volume-title":"survminer: Drawing Survival Curves using 'ggplot2'","year":"2020","author":"Kassambara A.","key":"e_1_2_8_25_1"},{"issue":"5","key":"e_1_2_8_26_1","first-page":"412","article-title":"Form and functions of the regular surface array (S\u2010layer) of Aeromonas salmonicida","volume":"47","author":"Kay W.","year":"1991","journal-title":"Experientia"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/are.12809"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.3.2_34"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.3.2_45"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks808"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2002.00407.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03006"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.36.3.1069-1075.1982"},{"issue":"1","key":"e_1_2_8_34_1","first-page":"571","article-title":"An exocrine pancreas disease of farmed Atlantic salmon in Scotland","volume":"37","author":"Munro A.","year":"1984","journal-title":"Helgolander Meeresuntersuchungen"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12237"},{"volume-title":"Manual of diagnostic tests for aquatic animals, Chapter 2.3","year":"2018","author":"OIE","key":"e_1_2_8_36_1"},{"volume-title":"Health screening and autogenous vaccination strategies for atypical Aeromonas salmonicida in farmed ballan wrasse (Labrus bergylta, Ascanius)","year":"2019","author":"Papadopoulou A.","key":"e_1_2_8_37_1"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03489"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-3-1119"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.09.040"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/fsim.1998.0162"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2005.01.012"},{"volume-title":"R: A language and environment for statistical computing","year":"2020","author":"R Core Team","key":"e_1_2_8_43_1"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-17-3-273"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.03.032"},{"issue":"2","key":"e_1_2_8_46_1","first-page":"263","article-title":"The classification of \u2018Bacterium salmonicida\u2019","volume":"33","author":"Smith I. W.","year":"1963","journal-title":"Microbiology"},{"volume-title":"A package for survival analysis in R","year":"2020","author":"Therneau T.","key":"e_1_2_8_47_1"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2012.01389.x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12099"},{"issue":"3","key":"e_1_2_8_50_1","first-page":"12","article-title":"Immunization of fish with bacterins of Aeromonas salmonicida","volume":"40","author":"Udey L.","year":"1978","journal-title":"Marine Fisheries Review"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.45.92"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-10-2385"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.00903.x"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.13312","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.13312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T14:57:12Z","timestamp":1693580232000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.13312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,4]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/jfd.13312"],"URL":"https:\/\/doi.org\/10.1111\/jfd.13312","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"type":"print","value":"0140-7775"},{"type":"electronic","value":"1365-2761"}],"subject":[],"published":{"date-parts":[[2020,12,4]]},"assertion":[{"value":"2020-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}