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Here, we conduct in vivo challenges of na\u00efve Atlantic salmon with cultured <jats:italic>Nolandella<\/jats:italic> sp. and <jats:italic>Pseudoparamoeba<\/jats:italic> sp. to investigate their pathogenicity to Atlantic salmon gills. Additionally, we assessed whether the presence of <jats:italic>Nolandella<\/jats:italic> sp. and <jats:italic>Pseudoparamoeba<\/jats:italic> sp. influences the onset and\/or severity of <jats:italic>N.\u00a0perurans<\/jats:italic>\u2010induced AGD. All three strains attached and multiplied on the gills according to qPCR analysis. Furthermore, minor gross gill lesions and histological changes were observed post\u2010exposure. While <jats:italic>N.\u00a0perurans<\/jats:italic> was found associated with classical AGD lesions, <jats:italic>Nolandella<\/jats:italic> sp. and <jats:italic>Pseudoparamoeba<\/jats:italic> sp. were not found associated with lesion sites and these lesions did not meet the expected composite of histopathological changes for AGD. Moreover, the presence of these non\u2010<jats:italic>N.\u00a0perurans<\/jats:italic> species did not significantly increase the severity of AGD. This trial provides evidence that cultured <jats:italic>Nolandella<\/jats:italic> sp. and <jats:italic>Pseudoparamoeba<\/jats:italic> sp. do not induce AGD and do not influence the severity of AGD during the early stages of development.<\/jats:p>","DOI":"10.1111\/jfd.13319","type":"journal-article","created":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T18:47:35Z","timestamp":1607798855000},"page":"149-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Immersion challenge of na\u00efve Atlantic salmon with cultured <i>Nolandella<\/i> sp. and <i>Pseudoparamoeba<\/i> sp. did not increase the severity of <i>Neoparamoeba perurans<\/i>\u2010induced amoebic gill disease (AGD)"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-493X","authenticated-orcid":false,"given":"Chloe J.","family":"English","sequence":"first","affiliation":[{"name":"School of Biological Sciences The University of Queensland Brisbane Qld Australia"},{"name":"Livestock &amp; Aquaculture CSIRO Bribie Island Research Centre Woorim Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha A.","family":"Botwright","sequence":"additional","affiliation":[{"name":"Livestock &amp; Aquaculture CSIRO Queensland Biosciences Precinct Brisbane Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark B.","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute for Marine and Antarctic Studies University of Tasmania Launceston Tas Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3990-8070","authenticated-orcid":false,"given":"Andrew C.","family":"Barnes","sequence":"additional","affiliation":[{"name":"School of Biological Sciences The University of Queensland Brisbane Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6846-757X","authenticated-orcid":false,"given":"James W.","family":"Wynne","sequence":"additional","affiliation":[{"name":"Livestock &amp; Aquaculture CSIRO Hobart Tas Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6621-662X","authenticated-orcid":false,"given":"Paula C.","family":"Lima","sequence":"additional","affiliation":[{"name":"Livestock &amp; Aquaculture CSIRO Queensland Biosciences Precinct Brisbane Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathew T.","family":"Cook","sequence":"additional","affiliation":[{"name":"Livestock &amp; Aquaculture CSIRO Queensland Biosciences Precinct Brisbane Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2012.01422.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2003.00496.x"},{"key":"e_1_2_8_4_1","volume-title":"The multifactorial aetiology of amoebic gill disease (AGD) in Ireland","author":"Bermingham M.","year":"2004"},{"issue":"3","key":"e_1_2_8_5_1","first-page":"234","article-title":"Neoparamoeba sp. and other protozoans on the gills of Atlantic salmon Salmo salar smolts in seawater","volume":"76","author":"Bermingham M.","year":"2007","journal-title":"Diseases of Aquatic Organisms"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skz325"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/raq.12426","article-title":"Epidemiology of marine gill diseases in Atlantic salmon (Salmo salar) aquaculture: A review","volume":"12","author":"Boerlage A. 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